Evaluation of peripheral basophil activation during exercise provocation test for desensitized patients

Jun Kunizaki; Shiro Sugiura; Akira Sakai; Miyuki Teshigawara; Atsushi Makino; Yoshihiro Takasato; Teruaki Matsui; Yasuto Kondo; Komei Ito

doi:10.3389/falgy.2023.1298137

Evaluation of peripheral basophil activation during exercise provocation test for desensitized patients

Front Allergy. 2023 Dec 22:4:1298137. doi: 10.3389/falgy.2023.1298137. eCollection 2023.

Authors

Jun Kunizaki^{1

2}, Shiro Sugiura¹, Akira Sakai^{1

3}, Miyuki Teshigawara⁴, Atsushi Makino¹, Yoshihiro Takasato¹, Teruaki Matsui¹, Yasuto Kondo⁴, Komei Ito¹

Affiliations

¹ Department of Allergy, Allergy and Immunology Center, Aichi Children's Health and Medical Center, Obu, Japan.
² Department of Pediatrics, NTT East Sapporo Hospital, Sapporo, Japan.
³ Department of Pediatrics, Hamamatsu Medical Center, Hamamatsu, Japan.
⁴ Department of Pediatrics, Bantane Hospital, Fujita Health University, Aichi, Japan.

Abstract

Some food allergic patients who have undergone oral immunotherapy develop exercise-induced allergic reactions on desensitization (EIARDs). This study investigated basophil activation status during the exercise provocation test (EPT) performed to diagnose EIARD. EPT was performed on 20 participants, and in vivo basophil activation status was analyzed using activation markers CD203c and CD63. The results showed that there was no significant difference between EPT-positive and negative subjects for basophil activation status throughout EPT. Consequently, in vivo basophil activation after ingestion of the causative food may not be associated with EIARDs. New tests are desired for predicting EIARDs.

Keywords: CD203c; CD63; basophil activation; exercise provocation test; exercise-induced allergic reactions on desensitization; oral immunotherapy.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article.