Metagenomic data of bacterial 16S rRNA in the cemetery soil samples in Surakarta City, Indonesia

Triastuti Rahayu; Erma Musbita Tyastuti; Ambarwati Ambarwati; Lina Agustina; Noor Alis Setiyadi; Nazia Jamil; Yasir Sidiq

doi:10.1016/j.dib.2023.109963

Metagenomic data of bacterial 16S rRNA in the cemetery soil samples in Surakarta City, Indonesia

Data Brief. 2023 Dec 14:52:109963. doi: 10.1016/j.dib.2023.109963. eCollection 2024 Feb.

Authors

Triastuti Rahayu¹, Erma Musbita Tyastuti¹, Ambarwati Ambarwati¹, Lina Agustina¹, Noor Alis Setiyadi², Nazia Jamil³, Yasir Sidiq¹

Affiliations

¹ Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Surakarta, Surakarta 57162, Indonesia.
² Department of Public Health, Faculty of Health Science, Universitas Muhammadiyah Surakarta, Surakarta 57162, Indonesia.
³ Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.

Abstract

Cemetery soils most likely contain degradative bacteria which possibly have beneficial potencies. However, the bacterial exploration in these potencies is still limitedly conducted in Indonesia. The raw sequence data of total bacteria in the cemetery soils through metagenomic analysis have been revealed. The data were obtained by collecting soil samples from six spots of two major Cemetery areas, which were Pracimaloyo (P) and Bonoloyo (B), in Surakarta City, Central Java, Indonesia. The six sample spots consisted of two samples from P area with respectively 20 cm and 140 cm depths and four samples of each two samples from B area with 20 and 40 cm depths. The total DNA was subsequently extracted from the collected soils using ZymoBIOMICS DNA Miniprep Kit. The total DNA then was amplified using a couple of 16S rRNA primers through Illumina HiSeq 2500 PE250 (Novogen, Korea) environment system. The raw sequence data has been submitted to the National Center for Biotechnology Information (NCBI) with project ID PRJNA997385. The archived sequence can be accessed in the NCBI website with the following URLs https://www.ncbi.nlm.nih.gov/sra/PRJNA997385. A brief analysis of the sequence data showed that the most common phyla in 20 cm-depths were Proteobacteria (29.5%), Actinobacteria (21.6%), and Firmicutes (19.2%), while Actinobacteria were the most found in 140 cm-depths with 34.2% followed by Proteobacteria (21.9%) and Firmicutes (16.6%). This data would be the first report of total bacterial sequence from cemetery soils in Indonesia.

Keywords: Bacteria; Cemetery soil; Genomic; Sequence data.