Identification and quantification of bipyridyl dicarboxylic acid isomers by ion mobility spectrometry

Abstract

The identification of positional isomers is of interest because different isomers have different chemical or biological functions and applications. The analysis of positional isomers is sometimes challenging since they have similar chemical structures and properties. For example, the analysis of mass cannot identify different positional isomers because they have identical mass-to-charge ratios and show a single mass peak in mass spectrometry. In this study, an efficient and simple qualitative and quantitative analytical method for differentiating 2,2'-bipyridine-3,3'-dicarboxylic acid (3,3'-BDA), 2,2'-bipyridine-4,4'-dicarboxylic acid (4,4'-BDA), and 2,2'-bipyridine-5,5'-dicarboxylic acid (5,5'-BDA) was developed by using ion mobility spectrometry (IMS). The results revealed that the three BDA isomers formed non-covalent complexes with cyclodextrins (CDs) and Mg²⁺ ions in the gas phase: [β-CD+3,3'/4,4'/5,5'-BDA+Mg]²⁺ and [γ-CD+3,3'/4,4'/5,5'-BDA+Mg]²⁺, which were distinguished by measuring the mobility of the complexes because of their spatial conformational differences. The peak-to-peak resolution (R_p-p) values of the three isomers of [γ-CD+3,3'/4,4'/5,5'-BDA+Mg]²⁺ reached 2.983 and 2.892, respectively. The conformations of the ternary complexes simulated by the theoretical calculations revealed the different interactions and shapes of the stereoisomers, and the predicted results agreed with the experimental results. Simultaneously, further studies on the collisional dissociation of the ternary complexes revealed that the dissociation energies of the different complex ions varied were different owing to the diverse different conformations. Finally, the relative quantitative analysis of the different isomers in mixed samples was performed and satisfactory linearity results (R² > 0.99) were obtained. Thus, an effective analytical method was proposed for the identification and quantification of BDA isomers without chemical derivatization, offering a promising approach for the identification of similar derivatives or positional isomers that could be applied in various fields including chemicals and pharmaceuticals.

Keywords: Bipyridine dicarboxylic acid; Identification and quantification; Ion mobility spectrometry; Non-covalent complex; Positional isomers.