Comparable detection of HPV using real-time PCR in paired cervical samples and concentrated first-stream urine collected with Colli-Pee device

Pornjarim Nilyanimit; Surasith Chaithongwongwatthana; Shina Oranratanaphan; Nimesh Poudyal; Jean-Louis Excler; Julia Lynch; Sompong Vongpunsawad; Yong Poovorawan

doi:10.1016/j.diagmicrobio.2023.116160

Comparable detection of HPV using real-time PCR in paired cervical samples and concentrated first-stream urine collected with Colli-Pee device

Diagn Microbiol Infect Dis. 2024 Mar;108(3):116160. doi: 10.1016/j.diagmicrobio.2023.116160. Epub 2023 Dec 12.

Authors

Pornjarim Nilyanimit¹, Surasith Chaithongwongwatthana², Shina Oranratanaphan², Nimesh Poudyal³, Jean-Louis Excler³, Julia Lynch³, Sompong Vongpunsawad⁴, Yong Poovorawan¹

Affiliations

¹ Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road Pathumwan, Bangkok 10330, Thailand.
² Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Thailand.
³ International Vaccine Institute, Seoul, South Korea.
⁴ Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road Pathumwan, Bangkok 10330, Thailand. Electronic address: sompong.vo@chula.ac.th.

Abstract

We compared high-risk human papillomavirus (HPV) detection on first-stream urine from self-sampled collection device (Colli-Pee) and same-day clinician-collected cervical swab in 240 women. Testing with automated cobas 4800 system showed 96.7 % concordance (198 concordant-negative, 34 concordant-positive, Cohen's kappa=0.87). HPV testing on Colli-Pee urine offers advantages for acceptable non-invasive HPV screening.

Keywords: Cervical swab; Cobas; Colli-Pee; Detection; HPV; Urine.

MeSH terms

DNA, Viral / analysis
DNA, Viral / genetics
Early Detection of Cancer
Female
Humans
Papillomaviridae / genetics
Papillomavirus Infections* / diagnosis
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Uterine Cervical Dysplasia* / diagnosis
Uterine Cervical Neoplasms* / diagnosis

Substances

DNA, Viral