Background: Microplastics have the capability of retaining contaminants on their surface, increasing their persistence, preconcentrating them, and acting as transport vectors. Nevertheless, the determination of these compounds in plastic matrices poses several analytical issues and challenges, including the capability of many of these methods of only determining the extractable pollutants fractions, repeatability issues, etc. In this sense, it is primordial to evaluate the effect of the critical parameters that allow to obtain a quantitative extraction of the target analytes from microplastics, including the matrix effect of each of the studied polymers, the influence of particle size, and the effect of weathering.
Results: A simple and effective methodology for the extraction of 17 emerging organic pollutants from both pristine (polypropylene, polystyrene, and low- and high-density polyethylene) and weathered (polypropylene and polyethylene) microplastics has been developed, optimized, and validated, achieving recovery values of 70-120 % and low method quantification limits (9.2-35.5 ng/g). Results show the importance of cryomilling microplastics (as smaller particle sizes improve recovery and homogenization), something ignored in most publications. The differences in matrix effect for the studied pristine polymers highlights the importance of treating polymers individually, without extrapolating results. In weathered microplastics, matrix effect is overall higher than in their pristine counterparts, evidencing the necessity of always carrying out matrix effect and recovery studies in environmental microplastics. The analysis of 10 samples collected in Playa Grande (Tenerife, Canary Islands, Spain) revealed quantitative amounts of bisphenol A (10.8 ± 3.4 ng/g) in one of them.
Significance: For the first time, the effect of particle size, weathering and matrix effect have been simultaneously evaluated on microplastics, revealing the importance of their assessment to properly validate the methodology. Additionally, the method shows good performance in all the different polymers and has been successfully applied to the analysis of environmental samples of microplastics.
Keywords: Contaminants; Cryomilling; Desorption; Microplastics; Sorption.
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