Titanium-based substrate modified with nanoenzyme for accelerating the repair of bone defect

Yingying Tan; Yao Ding; Shaopeng Liu; Peng Liu; Kaiyong Cai

doi:10.1016/j.colsurfb.2023.113737

Titanium-based substrate modified with nanoenzyme for accelerating the repair of bone defect

Colloids Surf B Biointerfaces. 2024 Feb:234:113737. doi: 10.1016/j.colsurfb.2023.113737. Epub 2023 Dec 29.

Authors

Yingying Tan¹, Yao Ding¹, Shaopeng Liu¹, Peng Liu², Kaiyong Cai³

Affiliations

¹ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing China.
² Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing China. Electronic address: liupeng79@cqu.edu.cn.
³ Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing China. Electronic address: kaiyong_cai@cqu.edu.cn.

PMID: 38176336
DOI: 10.1016/j.colsurfb.2023.113737

Abstract

Titanium (Ti) and titanium alloy are the most common metal materials in clinical orthopedic surgery. However, in the initial stage of surgery and implantation, the production of excessive reactive oxygen species (ROS) can induce oxidative stress (OS) microenvironment. OS will further inhibit the growth of new bone, resulting in surgical failure. In this study, based on the fact that nanoscale manganese dioxide (MnO₂) can show H₂O₂-like enzyme activity, a MnO₂ nanocoating was prepared on mciro-nano structured surface of Ti substrate via a two-step method of alkaline thermal and hydrothermal treatment. The results of scanning electron microscopy (SEM), X-ray diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS) showed that the nano-MnO₂ coating was successfully fabricated on the surface of Ti substrate. The results of measurement of H₂O₂, dissolved O₂ and intracellular ROS in vitro showed that the treated Ti substrate could efficiently eliminate H₂O₂ and reduce ROS. Furthermore, the modified Ti substrate could promote the early adhesion, proliferation and osteogenic differentiation of MSCs, which was demonstrated by experimental results of cell morphology, cell viability, alkaline phosphatase, collagen, and mineralization deposition. The results of quantitative real-time polymerase chain reaction (qRT-PCR) of MSCs adhered the modified Ti substrate showed that the expression of genes related to osteogenic differentiation significantly increased. More importantly, the modified Ti implant could eliminate ROS at the injury site, reduce OS and promote the regeneration of bone tissue, which was demonstrated via hematoxylin/eosin, Masson's trichrome and immunohistochemical staining. In conclusion, the modified Ti implant presented here had the effect of reducing OS and promoting osseointegration. Relevant research ideas and results provide new methods for the research and development of functional implants, which have potential application value in the field of orthopedics.

Keywords: Bone repair; Nanoenzyme; Oxidative stress; Surface modification; Titanium substrate.

MeSH terms

Hydrogen Peroxide / pharmacology
Manganese Compounds / pharmacology
Osseointegration
Osteogenesis*
Oxides / pharmacology
Reactive Oxygen Species / metabolism
Surface Properties
Titanium* / chemistry
Titanium* / pharmacology

Substances

Titanium
Manganese Compounds
Reactive Oxygen Species
Oxides
Hydrogen Peroxide