The existence of nanobubbles in pure water has been extensively debated in recent years, and it is speculated that nanobubbles may be ion-stabilized. However, nanobubbles in the alcohol-water mixture and pure alcohols are still controversial due to the lack of ions present in the alcohol system. This work tested the hypothesis that stable nanobubbles exist in pure alcohol. The ultrasound and oscillatory pressure fields are used to generate nanobubbles in pure alcohol. The size distribution, concentration, diameter, and scattering intensity of the nanobubbles were measured by nanoparticle tracking analysis. The light scattering method measures the zeta potential. The Mie scattering theory and electromagnetic wave simulation are utilized to estimate the refractive index (RI) of nanobubbles from the experimentally measured scattering light intensity. The average RI of the nanobubbles in pure alcohols produced by ultrasound and oscillating pressure fields was estimated to be 1.17 ± 0.03. Degassing the nanobubble sample reduces its concentration and increases its size. The average zeta potential of the nanobubbles in pure alcohol was measured to be -5 ± 0.9 mV. The mechanical stability model, which depends on force balance around a single nanobubble, also predicts the presence of nanobubbles in pure alcohol. The nanobubbles in higher-order alcohols were found to be marginally colloidally stable. In summary, both experimental and theoretical results suggest the existence of nanobubbles in pure alcohol.