Transgene-free CRISPR/Cas9-mediated gene editing through protoplast-to-plant regeneration enhances active compounds in Salvia miltiorrhiza
Plant Biotechnol J
.
2024 Jan 4.
doi: 10.1111/pbi.14285.
Online ahead of print.
Authors
Chen-Tran Hsu
1
,
Chi-Chou Chiu
2
,
Pao-Yuan Hsiao
1
,
Chih-Yu Lin
1
,
Sychyi Cheng
1
3
,
Yao-Cheng Lin
1
3
,
Yu-Liang Yang
1
3
,
Fu-Hui Wu
1
,
Horng-Jyh Harn
4
5
,
Shinn-Zong Lin
4
6
,
Choun-Sea Lin
1
Affiliations
1
Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
2
Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University, Tainan, Taiwan.
3
Biotechnology Research Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan.
4
Bioinnovation Center, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
5
Department of Pathology, Hualien Tzu Chi Hospital and Tzu Chi University, Hualien, Taiwan.
6
Department of Neurosurgery, Hualien Tzu Chi Hospital, Hualien, Taiwan.
PMID:
38174833
DOI:
10.1111/pbi.14285
No abstract available
Keywords:
CRISPR; protoplast regeneration; ribonucleoprotein.
Grants and funding
AS-KPQ-107-ITAR-107/Innovative Translational Agricultural Research Administrative Office, Academia Sinica
AS-KPQ-108-ITAR-108/Innovative Translational Agricultural Research Administrative Office, Academia Sinica
AS-KPQ-109-ITAR-109/Innovative Translational Agricultural Research Administrative Office, Academia Sinica
109-2313-B-001-011/Ministry of Science and Technology, Taiwan
105-2313-B-001-007-MY3/Ministry of Science and Technology, Taiwan
108-2313-B-001-011/Ministry of Science and Technology, Taiwan
110-2313-B-001-006-MY3/Ministry of Science and Technology, Taiwan
Buddhist Tzu Chi Medical Foundation, Taiwan