Stable organic π-conjugated diradcialoids with tunable diradical characters can profoundly affect emerging technology. Over the past years, great efforts have been devoted to studying the structure-diradical character relationship in diradicaloids. Herein, a series of quinoidal isoindigo (IID) compounds with different attached terminal end groups were designed. Detailed analysis focuses on elucidating the driving force for evoking and enhancing the diradical character in the quinoidal IID systems. The arylene units of the IID core and the bridged aromatic units determine the contribution of the open-shell diradical form in the ground state. Diradical character y0 correlates well with bond length alternation (BLA), the total HOMA, and the total NICS(1)zz, and it is tuned by bridged aromatic units and terminal end groups in symmetric systems. The zwitterionic character weakens the diradical character in asymmetric systems to different extents. This work contributes to the deep understanding of evoking and enhancing the diradical character in quinoidal IID-based diradcialoids, providing useful guidelines to produce new molecules with desirable properties.