A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples

Jiasheng Lin; Sanhua Li; Benchen Ye; Weigang Zheng; Huihui Wang; Ying Liu; Dong Wang; Zaihui Wu; Wen-Fei Dong; Minghui Zan

doi:10.3389/fbioe.2023.1308725

A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples

Front Bioeng Biotechnol. 2023 Dec 19:11:1308725. doi: 10.3389/fbioe.2023.1308725. eCollection 2023.

Authors

Jiasheng Lin^#^{1

2}, Sanhua Li^#³, Benchen Ye^{2

4}, Weigang Zheng^{2

4}, Huihui Wang^{2

4}, Ying Liu^{1

2}, Dong Wang^{1

2}, Zaihui Wu^{1

2}, Wen-Fei Dong^{1

2}, Minghui Zan^{1

2

4}

Affiliations

¹ CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China.
² Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
³ Henan Province Joint International Laboratory for Bioconjugation and Antibody Coupling, Zhengzhou, China.
⁴ Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China.

^# Contributed equally.

Abstract

Introduction: Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance. Methods: To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip. Results: The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30-2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%. Discussion: The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.

Keywords: Pregnanediol-3-glucuronide; competitive model; lateral flow immunochromatographic; time-resolved fluorescence microspheres; urine sample.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the National Key R&D Program of China (Grand No. 2022YFC2403500) and the China Postdoctoral Science Foundation (No. 2022T150464).