Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.
Keywords: 3-D cultures; Borrelia; Infectious disease; Lyme disease; Organoid.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.