GDF-10 Induces an Inhibitory Effect on Epithelial-Mesenchymal Transition of Laryngeal Cancer via LPR4

Curr Pharm Des. 2023;29(43):3467-3477. doi: 10.2174/0113816128261029231206035206.

Abstract

Background: Growth differentiation factor-10 (GDF-10), a member of the TGF-β superfamily, plays a crucial role in cell proliferation and differentiation. In some tumors, GDF-10 can act as a tumor suppressor to inhibit tumor progression, but its role in posterior squamous cell carcinoma has not been reported yet.

Methods: The aim of this study was to investigate the effect of GDF-10 on the epithelial-mesenchymal transition of laryngeal squamous cell carcinoma, and to provide new ideas for future targets in the treatment of laryngeal squamous carcinoma.

Results: The effect of GDF-10 on tumor growth was detected; bioinformatics analysis was performed to predict the downstream targets of GDF-10, and RT-PCR and western blot were performed to detect the expression levels of target genes and proteins, respectively.

Conclusion: Our findings support that GDF-10 can inhibit the proliferation, migration, and invasion, and promote apoptosis of laryngeal carcinoma AMC-HN-8 cells. GDF-10 inhibits the EMT of laryngeal carcinoma through LRP4 and thus inhibits the progression of laryngeal carcinoma.

Keywords: EMT; GDF-10; LRP4; Laryngeal cancer; TGF-β; tumor progression..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell* / drug therapy
  • Carcinoma, Squamous Cell* / genetics
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Epithelial-Mesenchymal Transition / genetics
  • Gene Expression Regulation, Neoplastic
  • Growth Differentiation Factor 10 / genetics
  • Growth Differentiation Factor 10 / metabolism
  • Humans
  • Laryngeal Neoplasms* / drug therapy
  • Laryngeal Neoplasms* / genetics
  • Laryngeal Neoplasms* / metabolism
  • Transforming Growth Factor beta / metabolism

Substances

  • Growth Differentiation Factor 10
  • Transforming Growth Factor beta