Marsh sediments chronically exposed to nitrogen enrichment contain degraded organic matter that is less vulnerable to decomposition via nitrate reduction

Ashley N Bulseco; Anna E Murphy; Anne E Giblin; Jane Tucker; Jonathan Sanderman; Jennifer L Bowen

doi:10.1016/j.scitotenv.2023.169681

Marsh sediments chronically exposed to nitrogen enrichment contain degraded organic matter that is less vulnerable to decomposition via nitrate reduction

Sci Total Environ. 2024 Mar 10:915:169681. doi: 10.1016/j.scitotenv.2023.169681. Epub 2023 Dec 30.

Authors

Ashley N Bulseco¹, Anna E Murphy², Anne E Giblin³, Jane Tucker³, Jonathan Sanderman⁴, Jennifer L Bowen⁵

Affiliations

¹ Marine Science Center, Northeastern University, Nahant, MA, USA; Department of Biological Sciences, University of New Hampshire, Durham, NH, USA.
² Marine Science Center, Northeastern University, Nahant, MA, USA; INSPIRE Environmental, Newport, RI, USA.
³ The Ecosystems Center, Marine Biological Laboratory, Woods Hole, MA, USA.
⁴ Woodwell Climate Research Center, Falmouth, MA, USA.
⁵ Marine Science Center, Northeastern University, Nahant, MA, USA. Electronic address: je.bowen@northeastern.edu.

PMID: 38163591
DOI: 10.1016/j.scitotenv.2023.169681

Abstract

Blue carbon habitats, including salt marshes, can sequester carbon at rates that are an order of magnitude greater than terrestrial forests. This ecosystem service may be under threat from nitrate (NO₃^-) enrichment, which can shift the microbial community and stimulate decomposition of organic matter. Despite efforts to mitigate nitrogen loading, salt marshes continue to experience chronic NO₃^- enrichment, however, the long-term consequence of this enrichment on carbon storage remains unclear. To investigate the effect of chronic NO₃^- exposure on salt marsh organic matter decomposition, we collected sediments from three sites across a range of prior NO₃^- exposure: a relatively pristine marsh, a marsh enriched to ~70 μmol L^-1 NO₃^- in the flooding seawater for 13 years, and a marsh enriched between 100 and 1000 μmol L^-1 for 40 years from wastewater treatment effluent. We collected sediments from 20 to 25 cm depth and determined that sediments from the most chronically enriched site had less bioavailable organic matter and a distinct assemblage of active microbial taxa compared to the other two sites. We also performed a controlled anaerobic decomposition experiment to test whether the legacy of NO₃^- exposure influenced the functional response to additional NO₃^-. We found significant changes to microbial community composition resulting from experimental NO₃^- addition. Experimental NO₃^- addition also increased microbial respiration in sediments collected from all sites. However, sediments from the most chronically enriched site exhibited the smallest increase, the lowest rates of total NO₃^- reduction by dissimilatory nitrate reduction to ammonium (DNRA), and the highest DNF:DNRA ratios. Our results suggest that chronic exposure to elevated NO₃^- may lead to residual pools of organic matter that are less biologically available for decomposition. Thus, it is important to consider the legacy of nutrient exposure when examining the carbon cycle of salt marsh sediments.

Keywords: Carbon storage; DNRA; Decomposition; Denitrification; Microbes; Nutrient enrichment; Sulfate reduction.

MeSH terms

Ammonium Compounds* / metabolism
Carbon / metabolism
Denitrification
Microbiota*
Nitrates / metabolism
Nitrogen / metabolism
Organic Chemicals
Wetlands

Substances

Nitrates
Nitrogen
Organic Chemicals
Ammonium Compounds
Carbon