An integrated plant glucose monitoring system based on microneedle-enabled electrochemical sensor

Han Chen; Shenghan Zhou; Junbiao Chen; Jin Zhou; Kai Fan; Yuxiang Pan; Jianfeng Ping

doi:10.1016/j.bios.2023.115964

An integrated plant glucose monitoring system based on microneedle-enabled electrochemical sensor

Biosens Bioelectron. 2024 Mar 15:248:115964. doi: 10.1016/j.bios.2023.115964. Epub 2023 Dec 21.

Authors

Han Chen¹, Shenghan Zhou², Junbiao Chen³, Jin Zhou⁴, Kai Fan⁵, Yuxiang Pan², Jianfeng Ping⁶

Affiliations

¹ Laboratory of Agricultural Information Intelligent Sensing, School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, PR China; Hangzhou Academy of Agricultural Sciences, Hangzhou, 310024, PR China.
² Laboratory of Agricultural Information Intelligent Sensing, School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, PR China; Innovation Platform of Micro/Nano Technology for Biosensing, ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311200, PR China.
³ College of Information Engineering, Hangzhou Dianzi University, Hangzhou, 310018, PR China.
⁴ Laboratory of Agricultural Information Intelligent Sensing, School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, PR China.
⁵ School of Automation, Hangzhou Dianzi University, Hangzhou, 310018, PR China.
⁶ Laboratory of Agricultural Information Intelligent Sensing, School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, PR China; Innovation Platform of Micro/Nano Technology for Biosensing, ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311200, PR China. Electronic address: jfping@zju.edu.cn.

PMID: 38160635
DOI: 10.1016/j.bios.2023.115964

Abstract

Real-time monitoring of glucose concentration changes in plants and access to plant physiological information timely are of great significance to the development of precision agriculture. Here, we innovatively present an electrochemical sensing device that combines microneedle sensors and 3D printing technology to achieve real-time monitoring of glucose in plants in a minimally invasive manner. The device consists of two components: the inner part features a highly efficient sensing interface based on platinum wire (MPt-Au-Nafion-GOx-Pu), while the outer part consists of polymer microneedles formed by 3D printing. Additionally, the polymer hollow microneedle features a slender tip diameter of only 300 μm, minimizing plant damage during the detection procedure. The device shows good detection performance, with a limit of detection (LOD) of 33.3 μM and a detection sensitivity of 17 nA/μM·cm². It can detect glucose concentrations in the range of 100 μM to 100 mM, providing a unique solution for timely agronomic management of crops tool. By performing 12 h real-time monitoring and salt stress treat on tomato and aloe vera, the results verified the feasibility of integrated device applied to real-time glucose detection in plants.

Keywords: Electrochemistry; Glucose; In situ sensing; Minimally invasive; Plant.

MeSH terms

Biosensing Techniques* / methods
Blood Glucose
Blood Glucose Self-Monitoring
Glucose*
Needles

Substances

Glucose
Blood Glucose