Efficacy of rhizobacteria Paenibacillus polymyxa SY42 for the biological control of Atractylodes chinensis root rot

Siyuan Xie; He Si; Yuyan Xue; Ru Zhou; Shiqiang Wang; Yizhong Duan; Junfeng Niu; Zhezhi Wang

doi:10.1016/j.micpath.2023.106517

Efficacy of rhizobacteria Paenibacillus polymyxa SY42 for the biological control of Atractylodes chinensis root rot

Microb Pathog. 2024 Feb:187:106517. doi: 10.1016/j.micpath.2023.106517. Epub 2023 Dec 29.

Authors

Siyuan Xie¹, He Si², Yuyan Xue³, Ru Zhou⁴, Shiqiang Wang⁵, Yizhong Duan⁶, Junfeng Niu⁷, Zhezhi Wang⁸

Affiliations

¹ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: xsy3094@snnu.edu.cn.
² National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: sihe1998@snnu.edu.cn.
³ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: 20213147@snnu.edu.cn.
⁴ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: zhouru0326@snnu.edu.cn.
⁵ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: wsq@snnu.edu.cn.
⁶ College of Life Sciences, Yulin University, Yulin, Shaanxi, 718000, China. Electronic address: duanyizhong2006@163.com.
⁷ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: niujunfeng@snnu.edu.cn.
⁸ National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an, 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address: zzwang@snnu.edu.cn.

PMID: 38159617
DOI: 10.1016/j.micpath.2023.106517

Abstract

Atractylodes chinensis is one of the most commonly used bulk herbs in East Asia; however, root rot can seriously affect its quality and yields. In contrast to chemical pesticides, biological control strategies are environmentally compatible and safe. For this study, 68 antagonistic bacterial strains were isolated from the rhizospheres of healthy Atractylodes chinensis. Strain SY42 exhibited the most potent fungicidal activities, with inhibition rates against F. oxysporum, F. solani, and F. redolens of 67.07 %, 63.40 % and 68.45 %, respectively. Through morphological observation and molecular characterization, strain SY42 was identified as Paenibacillus polymyxa. The volatile organic components (VOCs) produced by SY42 effectively inhibited the mycelial growth of pathogenic fungi through diffusion. SY42 significantly inhibited the germination of pathogenic fungal spores. Following co-culturing with SY42, the mycelium of the pathogenic fungus was deformed, folded, and even ruptured. SY42 could produce cellulases and proteases to degrade fungal cell walls. Pot experiments demonstrated the excellent biocontrol efficacy of SY42. This study revealed that P. polymyxa SY42 inhibited pathogenic fungi through multiple mechanisms, which verified its utility as a biocontrol agent for the control of A. chinensis root rot.

Keywords: Atractylodes chinensis; Biological control; Paenibacillus polymyxa; Rhizobacteria; Root rot.

MeSH terms

Atractylodes*
Fusarium*
Mycelium
Paenibacillus polymyxa*
Plant Diseases / microbiology
Plant Diseases / prevention & control