Synergistic influence of selenium and silicon supplementation prevents the oxidative effects of arsenic stress in wheat

Abstract

Influence of supplementation of selenium (Se, 1 and 5 µM) and silicon (Si, 0.1 and 0.5 mM) was investigated in wheat under arsenic (30 µM As) stress. Plants grown under As stress exhibited a significant decline in growth parameters however, Se and Si supplementation mitigated the decline significantly. Treatment of Se and Si alleviated the reduction in the intermediate components of chlorophyll biosynthesis pathway and the content of photosynthetic pigments. Arsenic stressed plants exhibited increased reactive oxygen species accumulation and the NADPH oxidase activity which were lowered significantly due to Se and Si treatments. Moreover, Se and Si supplementation reduced lipid peroxidation and activity of lipoxygenase and protease under As stress. Supplementation of Se and Si significantly improved the antioxidant activities and the content of cysteine, tocopherol, reduced glutathione and ascorbic acid. Treatment of Se and Si alleviated the reduction in nitrate reductase activity. Exogenously applied Se and Si mitigated the reduction in mineral elements and reduced As accumulation. Hence, supplementation of Se and Si is beneficial in preventing the alterations in growth and metabolism of wheat under As stress.

Keywords: Antioxidants; Arsenic; NADPH oxidase; Osmolytes; Redox components; Selenium; Silicon; Wheat.