Neutrophil-Derived MicroRNA-1290 Promotes Keratinocyte Proliferation in Psoriasis

Yuko Higashi; Munekazu Yamakuchi; Atsuko Ibusuki; Aoi Okubo; Tomoko Fukushige; Teruto Hashiguchi; Takuro Kanekura

doi:10.1016/j.jid.2023.10.042

Neutrophil-Derived MicroRNA-1290 Promotes Keratinocyte Proliferation in Psoriasis

J Invest Dermatol. 2023 Dec 28:S0022-202X(23)03209-8. doi: 10.1016/j.jid.2023.10.042. Online ahead of print.

Authors

Yuko Higashi¹, Munekazu Yamakuchi², Atsuko Ibusuki³, Aoi Okubo³, Tomoko Fukushige³, Teruto Hashiguchi⁴, Takuro Kanekura³

Affiliations

¹ Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: higashiy@m.kufm.kagoshima-u.ac.jp.
² Department of Laboratory and Vascular Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: k8412148@m.kufm.kagoshima-u.ac.jp.
³ Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
⁴ Department of Laboratory and Vascular Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.

PMID: 38157932
DOI: 10.1016/j.jid.2023.10.042

Abstract

The pathological hallmark of psoriasis is the infiltration of neutrophils into the skin. Some neutrophil-derived microRNAs (miRNAs) serve as biomarkers for various diseases, but none have been reported for psoriasis. In this study, we investigated the involvement of miRNAs released from neutrophils in psoriasis pathogenesis. We compared the expression of miRNAs in the sera of patients with psoriasis with that in healthy individuals and found that the expression of 2 miRNAs-miR-223 and miR-1290-was significantly upregulated in the sera of patients with psoriasis. The serum levels of these miRNAs positively correlated with the PASI and CRP levels. We used all-trans retinoic acid to induce the differentiation of human promyelocytic leukemia HL-60 cells into neutrophil-like cells and found that the release of both miRNAs increased during differentiation. Furthermore, the release of miR-1290 was increased by TNF-α in neutrophil-like cells and human neutrophils. Treatment with the miR-1290 precursor promoted the proliferation of human keratinocytes, increased the proportion of S-phase cells, and upregulated the phosphorylation of extracellular signal-regulated kinase 1/2. These results suggest that miR-1290 plays a vital role in regulating neutrophil differentiation and keratinocyte proliferation and could be a serum marker of psoriasis severity.

Keywords: psoriasis, neutrophil, microRNA, proliferation, keratinocyte.