Structurally diverse stilbenes from Gnetum parvifolium and their anti-neuroinflammatory activities

Abstract

Phytochemical investigation on the aerial parts of Gnetum parvifolium led to the isolation of 15 new and eight known structurally diverse stilbenes. The isolated compounds comprised (E)- or (Z)-stilbene (1-6, 15-20), dihydrostilbene (21), phenylbenzofuran (7, 8, 22), benzylated stilbene (9-11), benzylated stilbene dimer (12), and nitrogen-containing stilbene (13a, 13b, 14) types. The structures of the new compounds (1-12, 13a, 13b, 14) were established through spectroscopic analyses and experimental and calculated ECD data. Compound 12 is the first stilbene dimer connected through a benzyl group. In the anti-neuroinflammatory activity assay, compounds 4, 5, 9-11, 13b, and 16-21 displayed significant inhibitory effects against LPS-induced NO release in BV-2 microglial cells, with IC₅₀ values of 0.35-16.1 μM. Compound 10 had the most potent activity (IC₅₀ = 0.35 μM), and the further research indicated that it could decrease the mRNA levels of iNOS, IL-1β, IL-6, and TNF-α in a dose-dependent manner.

Keywords: Anti-neuroinflammatory; Gnetaceae; Gnetum parvifolium; Stilbenoids.