Biological control of potato common scab and growth promotion of potato by Bacillus velezensis Y6

Huan Tao; Shisong Wang; Xiaoyu Li; Xiaobo Li; Jianying Cai; Lanfeng Zhao; Jia Wang; Ji Zeng; Yuzhi Qin; Xingyao Xiong; Yanfei Cai

doi:10.3389/fmicb.2023.1295107

Biological control of potato common scab and growth promotion of potato by Bacillus velezensis Y6

Front Microbiol. 2023 Dec 11:14:1295107. doi: 10.3389/fmicb.2023.1295107. eCollection 2023.

Authors

Huan Tao^#¹, Shisong Wang^#¹, Xiaoyu Li^#¹, Xiaobo Li², Jianying Cai¹, Lanfeng Zhao¹, Jia Wang³, Ji Zeng⁴, Yuzhi Qin⁵, Xingyao Xiong⁶, Yanfei Cai¹

Affiliations

¹ College of Natural Resources and Environment, South China Agricultural University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
³ Guangdong Institute Center of Wine and Spirits, Guangdong Institute of Food Inspection, Guangzhou, China.
⁴ Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China.
⁵ Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education Changsha, Hunan Provincial Engineering Research Center for Potatoes, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China.
⁶ Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

^# Contributed equally.

Abstract

Potato common scab, caused mainly by Streptomyces scabies, causes surface necrosis and reduces the economic value of potato tubers, but effective chemical control is still lacking. In this study, an attempt was made to control potato common scab by inoculating potatoes with Bacillus velezensis (B. velezensis) and to further investigate the mechanism of biological control. The results showed that B. velezensis Y6 could reduce the disease severity of potato common scab from 49.92 ± 25.74% [inoculated with Streptomyces scabies (S. scabies) only] to 5.56 ± 1.89% (inoculated with S. scabies and Y6 on the same day) and increase the potato yield by 37.32% compared with the control under pot experiment in this study. Moreover, in the field trial, it was found that Y6 could also significantly reduce disease severity from 13.20 ± 1.00% to 4.00 ± 0.70% and increase the potato yield from 2.07 ± 0.10 ton/mu to 2.87 ± 0.28 ton/mu (p < 0.01; Tukey's test). Furthermore, RNA-seq analysis indicated that 256 potato genes were upregulated and 183 potato genes were downregulated in response to B. velezensis Y6 inoculation. In addition, strain Y6 was found to induce the expression of plant growth-related genes in potato, including cell wall organization, biogenesis, brassinosteroid biosynthesis, and plant hormone transduction genes, by 1.01-4.29 times. As well as up-regulate hydroquinone metabolism-related genes and several transcription factors (bHLH, MYB, and NAC) by 1.13-4.21 times. In summary, our study will help to understand the molecular mechanism of biological control of potato common scab and improve potato yield.

Keywords: Bacillus velezensis; Streptomyces scabies; antagonism; lipopeptide; potato; transcriptome.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the National Natural Science Foundation of China (41977035).