Genome-wide analysis of the TIFY family in Lycium and the negative regulation of stomatal development by LrJAZ2 gene

Jiqing Zhao; Aijia Li; Meng Xu; Guoli Dai; Jinhuan Chen

doi:10.1016/j.plaphy.2023.108285

Genome-wide analysis of the TIFY family in Lycium and the negative regulation of stomatal development by LrJAZ2 gene

Plant Physiol Biochem. 2024 Jan:206:108285. doi: 10.1016/j.plaphy.2023.108285. Epub 2023 Dec 20.

Authors

Jiqing Zhao¹, Aijia Li², Meng Xu³, Guoli Dai⁴, Jinhuan Chen⁵

Affiliations

¹ College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing, 100083, China; National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China.
² College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; International Center for Bamboo and Rattan, Beijing, 100102, China.
³ College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
⁴ National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002, China.
⁵ College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing, 100083, China; National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China. Electronic address: chenjh@bjfu.edu.cn.

PMID: 38145586
DOI: 10.1016/j.plaphy.2023.108285

Abstract

Stomata are ports that facilitate gas and water vapor exchange during plant photosynthesis and transpiration. Stomatal development is strictly regulated by endogenous hormone. Jasmonate, an important signal that modulates multiple physiological processes in plants, has been found to negatively regulate stomatal development in Arabidopsis thaliana, yet the molecular mechanisms underlying stomata development signaling remain to be understood. Jasmonate ZIM-domain (JAZ) proteins are the members of TIFY family and the key component of JA signaling pathway. Its function in stomatal development is unclear to data. Here, we screened out 24 TIFY family members against the genome of Lycium, and identified a JAZ member by combination analyses of evolutionary tree, cis-elements in promoter and gene expression patterns. Overexpression of this gene (LrJAZ2) in Lycium ruthenicum and Arabidopsis thaliana indicated LrJAZ2 negatively regulates stomatal development. Microscopic observations revealed that overexpression of LrJAZ2 negatively regulated stomatal development by decreasing stomatal density and index, which may lead to lower leaf transpiration rates. Transcriptome data indicated the overexpression of LrJAZ2 up-regulated the stomatal related genes such as LrERL2, LrPYL4, and down-regulated the LrSPCH. Collectively, our study found that LrJAZ2 is a key gene in stomatal development regulation in L. ruthenicum and provided new insights into the regulation of stomatal development.

Keywords: Jasmonic acid; LrJAZ2; Lycium; Stomatal development; TIFY family.

MeSH terms

Arabidopsis Proteins* / genetics
Arabidopsis* / genetics
Arabidopsis* / metabolism
Cyclopentanes / metabolism
Gene Expression Regulation, Plant
Lycium* / genetics
Oxylipins / metabolism

Substances

jasmonic acid
Cyclopentanes
Oxylipins
Arabidopsis Proteins