Proteomic and metabolomic analysis of ageing beef exudate to determine that iron metabolism enhances muscle protein and lipid oxidation

Jun Liu; Cuili Pan; Hui Yue; He Li; Dunhua Liu; Ziying Hu; Yuanliang Hu; Xiang Yu; Weiwei Dong; Yanli Feng

doi:10.1016/j.fochx.2023.101038

Proteomic and metabolomic analysis of ageing beef exudate to determine that iron metabolism enhances muscle protein and lipid oxidation

Food Chem X. 2023 Nov 30:20:101038. doi: 10.1016/j.fochx.2023.101038. eCollection 2023 Dec 30.

Authors

Jun Liu^{1

2

3}, Cuili Pan^{4

3}, Hui Yue¹, He Li¹, Dunhua Liu³, Ziying Hu³, Yuanliang Hu^{1

2}, Xiang Yu^{1

2}, Weiwei Dong¹, Yanli Feng¹

Affiliations

¹ Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
² Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi 435002, China.
³ Faculty of Life and Food Sciences, Ningxia University, 750021 Yinchuan, China.
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Abstract

The study aimed to assess differences in proteomic and metabolite profiles in ageing (1, 2, 4, and 6 days at 4 °C) beef exudates and determine their relationship with beef muscle iron metabolism and oxidation. Proteomic and metabolomic analyses identified 877 metabolites and 1957 proteins. The joint analysis identified 24 differential metabolites (DMs) and 56 differentially expressed proteins (DEPs) involved in 15 shared pathways. Ferroptosis was identified as the only iron metabolic pathway, and 4 DMs (l-glutamic acid, arachidonic acid, glutathione and gamma-glutamylcysteine) and 5 DEPs (ferritin, phospholipid hydroperoxide glutathione peroxidase, heme oxygenase 1, major prion protein, and acyl-CoA synthetase long chain family member 4) were involved in iron metabolism by regulating heme and ferritin degradation, Fe²⁺ and Fe³⁺ conversion, arachidonic acid oxidation and inactivation of glutathione peroxidase (GPX) 4, leading to increased levels of free iron, ROS, protein and lipid oxidation (P < 0.05). Overall, abnormal iron metabolism during ageing induced oxidative stress in muscle tissue.

Keywords: Ageing; Beef; Exudate; Iron metabolism; Oxidation.