miR-133b Promotes Esophageal Squamous Cell Carcinoma Metastasis

Cong-Gai Huang; Qing Liu; Shu-Tao Zheng; Tao Liu; Yi-Yi Tan; Tian-Yuan Peng; Jiao Chen; Xiao-Mei Lu

doi:10.1177/11795549231219502

miR-133b Promotes Esophageal Squamous Cell Carcinoma Metastasis

Clin Med Insights Oncol. 2023 Dec 22:17:11795549231219502. doi: 10.1177/11795549231219502. eCollection 2023.

Authors

Cong-Gai Huang^{1

2}, Qing Liu¹, Shu-Tao Zheng¹, Tao Liu¹, Yi-Yi Tan¹, Tian-Yuan Peng¹, Jiao Chen¹, Xiao-Mei Lu¹

Affiliations

¹ State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
² Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, P R China.

Abstract

Background: Evaluation of biological changes at the molecular level has important clinical implications for improving the survival rate of esophageal squamous cell carcinoma (ESCC). Therefore, we plan to analyze and elucidate the expression of microRNA-133b (miR-133b), M2 pyruvate kinase (PKM2), and signal transducer and activator of transcription 3 (STAT3) in ESCC and their associated clinicopathological significance.

Methods: The 72 patients with ESCC were selected as the experimental study group. Normal adjacent tissues (NAT) were matched as the control group. In this study, in situ hybridization was used to detect the expression of miR-133b in ESCC, and tissue expressions of PKM2 and STAT3 were detected by immunohistochemistry, and literature review was conducted.

Results: Studies had shown that the positive expression of miR-133b in NAT was significantly higher than that in ESCC (χ² = 9.007, P = .003). PKM2 and STAT3 in ESCC had a significantly higher positive expression levels than those of NAT (χ² = 56.523, P = .000; χ² = 72.939, P = .000). From correlation analysis, there was a negative correlation between miR-133b and PKM2(r = -0.515, P < .001), a negative correlation between miR-133b and STAT3(r = -0.314, P = .007), and a positive correlation between PKM2 and STAT3(r = 0.771, P < .001).

Conclusions: In ESCC, our study demonstrated that downregulation of miR-133b and upregulation of PKM2 and STAT3. We predict that miR-133b may inhibit the STAT3 pathway by downregulating PKM2.

Keywords: Esophageal squamous cell carcinoma; PKM2; STAT3; clinicopathological characteristics; miR-133b.