Current detection methods of African swine fever virus

Zhiqiang Hu; Xiaogang Tian; Ranran Lai; Xinglong Wang; Xiaowen Li

doi:10.3389/fvets.2023.1289676

Current detection methods of African swine fever virus

Front Vet Sci. 2023 Dec 8:10:1289676. doi: 10.3389/fvets.2023.1289676. eCollection 2023.

Authors

Zhiqiang Hu^#^{1

2

3

4}, Xiaogang Tian^#^{1

2

3}, Ranran Lai^#^{1

2

3}, Xinglong Wang⁵, Xiaowen Li^{1

2

3

4

5

6}

Affiliations

¹ Shandong Engineering Laboratory of Pig and Poultry Healthy Breeding and Disease Diagnosis Technology, Xiajin New Hope Liuhe Agriculture and Animal Husbandry Co., Ltd., Dezhou, China.
² Shandong New Hope Liuhe Co., Ltd., Qingdao, China.
³ Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy of Swine Research), Dezhou, China.
⁴ China Agriculture Research System-Yangling Comprehensive Test Station, Xianyang, China.
⁵ College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
⁶ Key Laboratory of Feed and Livestock and Poultry Products Quality and Safety Control, Ministry of Agriculture and Rural Affairs, New Hope Liuhe Co., Ltd., Chengdu, China.

^# Contributed equally.

Abstract

African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious and notifiable animal disease in domestic pigs and wild boars, as designated by the World Organization for Animal Health (WOAH). The effective diagnosis of ASF holds great importance in promptly controlling its spread due to its increasing prevalence and the continuous emergence of variant strains. This paper offers a comprehensive review of the most common and up-to-date methods established for various genes/proteins associated with ASFV. The discussed methods primarily focus on the detection of viral genomes or particles, as well as the detection of ASFV associated antibodies. It is anticipated that this paper will serve as a reference for choosing appropriate diagnostic methods in diverse application scenarios, while also provide direction for the development of innovative technologies in the future.

Keywords: African swine fever virus; antibodies; detection method; laboratory testing; on-site testing; viral genomes.

Publication types

Review

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by Taishan Industry Leadership Talent Project of Shandong Province in China, and the earmarked fund for CARS (CARS-35) and National Key R&D Program of China (2021ZD0113800).