Influence of Cordyceps militaris-fermented grain substrate extracts on alleviating food allergy in mice

Jia-Shan Liu; Rong-Yi Huang; Yu-Jyun Wei; Guo-Jane Tsai; Chung-Hsiung Huang

doi:10.1016/j.heliyon.2023.e23315

Influence of Cordyceps militaris-fermented grain substrate extracts on alleviating food allergy in mice

Heliyon. 2023 Dec 5;9(12):e23315. doi: 10.1016/j.heliyon.2023.e23315. eCollection 2023 Dec.

Authors

Jia-Shan Liu¹, Rong-Yi Huang¹, Yu-Jyun Wei¹, Guo-Jane Tsai^{1

2}, Chung-Hsiung Huang^{1

2}

Affiliations

¹ Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan.
² Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.

Abstract

Background: Cordyceps militaris is recognized as a tonic in traditional Chinese medicine, and there have been documented findings on the anti-allergic properties of its extract derived from the fruiting body. Due to the limited availability of wild C. militaris, a specialized grain substrate has been devised for the solid-state fermentation of its fruiting bodies. However, the fermented grain substrate is considered waste and usually used as feeds for animals. To achieve the sustainable development goals, C. militaris-fermented grain substrate (CFGS) was collected to prepare CFGS extracts. Further, the anti-allergic properties of these extracts were assessed with the aim of exploring novel applications.

Methods: The water extract and ethanol extract of CFGS were prepared, and their potential in alleviating allergic enteritis was assessed in mice with food allergy. Assessment of immunomodulatory effects included the measurement of serum antibodies and splenic cytokines. Additionally, influence of extracts on gut microbiota composition was examined through sequencing analysis of 16S rRNA gene from freshly collected feces of the mice.

Results: Daily administration of the water and ethanol extracts, at doses of 50 or 250 mg/kg body weight, demonstrated a notable alleviation of allergic diarrhea and enteritis. This was accompanied by a decrease in mast cell infiltration in the duodenum and a reduction in allergen-specific IgE production in the serum. Both extracts led to a significant decrease in IL-4 secretion. Conversely, there was an increase in IFN-γ, IL-10, and TGF-β secretion from splenocytes. Remarkably, allergic mice exhibited a distinct fecal microbiota profile compared to that of normal mice. Intriguingly, the administration of these extracts had varying effects on the fecal microbiota.

Conclusion: Taken together, these findings collectively indicate the potential of CFGS extracts as promising candidates for functional foods. These extracts show promise in managing allergic enteritis and modulating gut microbiota.

Keywords: Allergic enteritis; Cordyceps militaris; Food allergy; Grain substrate; Gut microbiota; Solid-state fermentation.