Antibody response to mycobacterial Rpf B protein and its immunodominant peptides in HIV-TB co-infected individuals

Madhur Kalyan; Sumedha Sharma; Prabhdeep Kaur; Aman Sharma; Indu Verma

doi:10.1016/j.tube.2023.102464

Antibody response to mycobacterial Rpf B protein and its immunodominant peptides in HIV-TB co-infected individuals

Tuberculosis (Edinb). 2024 Jan:144:102464. doi: 10.1016/j.tube.2023.102464. Epub 2023 Dec 16.

Authors

Madhur Kalyan¹, Sumedha Sharma², Prabhdeep Kaur³, Aman Sharma⁴, Indu Verma⁵

Affiliations

¹ Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: mdhrkalyan@gmail.com.
² Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: sumedha.pu@gmail.com.
³ Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: prabhdeep9361@gmail.com.
⁴ Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: amansharma74@yahoo.com.
⁵ Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: induvermabio@gmail.com.

PMID: 38141523
DOI: 10.1016/j.tube.2023.102464

Abstract

Diagnosis of TB at early stages of HIV infection may lead to timely intervention for improving patient outcome. Antibodies to Mycobacterium tuberculosis recombinant RpfB protein and two immunodominant peptides of Rpf B protein were evaluated in the sera of HIV ⁺TB⁺, HIV⁺ and HIV^- pulmonary TB patients by ELISA. Serum antibodies from 90 % and 65 % of HIV⁺TB⁺ patients reacted to recombinant RpfB protein and synthetic peptide RpfP1 respectively. Overall, this study shows that resuscitation promoting factor B elicits humoral antibody response in HIV⁺TB⁺ co-infected individuals and be proposed as a potential biomarker for diagnosis of HIV⁺TB⁺ patients, however further longitudinal follow up studies are warranted.

Keywords: Enzyme-linked immunosorbent assay; Extrapulmonary tuberculosis; HIV; Peptide; Pulmonary tuberculosis; Resuscitation promoting factors; Tuberculosis.

MeSH terms

Antibody Formation
Antigens, Bacterial
Coinfection*
Enzyme-Linked Immunosorbent Assay
HIV Infections* / complications
HIV Infections* / diagnosis
Humans
Mycobacterium tuberculosis*
Peptides

Substances

Peptides
Antigens, Bacterial