Composition and structure analysis of different depths in the stratum corneum using confocal Raman microscopy combined with two-dimensional correlation spectroscopy

Feng Zhong; Feng Wang; Haifeng Yang

doi:10.1016/j.talanta.2023.125559

Composition and structure analysis of different depths in the stratum corneum using confocal Raman microscopy combined with two-dimensional correlation spectroscopy

Talanta. 2024 Apr 1:270:125559. doi: 10.1016/j.talanta.2023.125559. Epub 2023 Dec 19.

Authors

Feng Zhong¹, Feng Wang², Haifeng Yang³

Affiliations

¹ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China.
² The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China. Electronic address: wangfeng@shnu.edu.cn.
³ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China. Electronic address: hfyang@shnu.edu.cn.

PMID: 38141465
DOI: 10.1016/j.talanta.2023.125559

Abstract

The chemical composition and structure of the stratum corneum (SC) play a crucial role in the skin barrier function. Therefore, accurately determining the SC thickness and studying the changes in lipid and keratin structure and distribution within it are key aspects of skin barrier research. Currently, there are limited analytical tools and data analysis methods available for real-time and online studies of SC composition and structural changes. In this study, we focus on depth as a perturbation and employ confocal Raman microscopy combined with moving-window two-dimensional correlation spectroscopy (MW2D) technique to investigate the SC thickness. Additionally, we employ confocal Raman microscopy combined with perturbation-correlation moving-window two-dimensional correlation spectroscopy (PCMW2D) to precisely characterize the stratification of the SC. Furthermore, the two-dimensional correlation spectroscopy (2DCOS) method is utilized to examine the content of various conformations in the keratin secondary structure within the SC, as well as the subtle interrelationships between lipid and keratin structures.

Keywords: 2DCOS; Composition; Confocal Raman imaging; Distribution; Stratum corneum; Stratum corneum thickness.

MeSH terms

Epidermis*
Keratins
Lipids
Microscopy, Confocal / methods
Skin
Spectrum Analysis, Raman* / methods

Substances

Keratins
Lipids