Genome-Wide Identification of AhMDHs and Analysis of Gene Expression under Manganese Toxicity Stress in Arachis hypogaea

Genes (Basel). 2023 Nov 21;14(12):2109. doi: 10.3390/genes14122109.

Abstract

Malate dehydrogenase (MDH) is one kind of oxidation-reduction enzyme that catalyzes the reversible conversion of oxaloacetic acid to malic acid. It has vital functions in plant development, photosynthesis, abiotic stress responses, and so on. However, there are no reports on the genome-wide identification and gene expression of the MDH gene family in Arachis hypogaea. In this study, the MDH gene family of A. hypogaea was comprehensively analyzed for the first time, and 15 AhMDH sequences were identified. According to the phylogenetic tree analysis, AhMDHs are mainly separated into three subfamilies with similar gene structures. Based on previously reported transcriptome sequencing results, the AhMDH expression quantity of roots and leaves exposed to manganese (Mn) toxicity were explored in A. hypogaea. Results revealed that many AhMDHs were upregulated when exposed to Mn toxicity, suggesting that those AhMDHs might play an important regulatory role in A. hypogaea's response to Mn toxicity stress. This study lays foundations for the functional study of AhMDHs and further reveals the mechanism of the A. hypogaea signaling pathway responding to high Mn stress.

Keywords: Arachis hypogaea; MDH; expression analysis; genome-wide identification; manganese toxicity stress.

MeSH terms

  • Arachis* / metabolism
  • Gene Expression
  • Genome, Plant / genetics
  • Manganese* / metabolism
  • Manganese* / toxicity
  • Phylogeny

Substances

  • Manganese

Grants and funding

This research was funded by the Project of Marine Young Talent Innovation of Zhanjiang City (210818004542097), the Project of Rural Science and Technology Team “One-to-One” Service to Help the Town Full Coverage of Action Funding in Zhanjiang City (A23081), the Postgraduate Education Innovation Project of Guangdong Ocean University-Excellent Dissertation Cultivation Project (040502752327), the Project of Social Services of Rural Science and Technology Specialists of Guangdong Ocean University (080503052212), the National Natural Science Foundation of China (32002131), and the Maintenance Expenses of Key Construction Disciplines in High-level University of Crop Science (080508052201).