Longitudinal Analysis of Canine Oral Microbiome Using Whole Genome Sequencing in Aging Companion Dogs

Ginger B Templeton; Gilad Fefer; Beth C Case; Jeff Roach; M Andrea Azcarate-Peril; Margaret E Gruen; Benjamin J Callahan; Natasha J Olby

doi:10.3390/ani13243846

Longitudinal Analysis of Canine Oral Microbiome Using Whole Genome Sequencing in Aging Companion Dogs

Animals (Basel). 2023 Dec 14;13(24):3846. doi: 10.3390/ani13243846.

Authors

Ginger B Templeton¹, Gilad Fefer¹, Beth C Case¹, Jeff Roach², M Andrea Azcarate-Peril², Margaret E Gruen¹, Benjamin J Callahan^{3

4}, Natasha J Olby¹

Affiliations

¹ Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
² Department of Medicine, Division of Gastroenterology and Hepatology, and UNC Microbiome Core, Center for Gastrointestinal Biology and Disease, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA.
³ Department of Population Health and Pathobiology, North Carolina State University, Raleigh, NC 27607, USA.
⁴ Bioinformatics Research Center, North Carolina State University, Raleigh, NC 27695, USA.

Abstract

Aged companion dogs have a high prevalence of periodontal disease and canine cognitive dysfunction syndrome (CCDS) and the two disorders are correlated. Similarly, periodontal disease and Alzheimer's Disease are correlated in people. However, little is known about the oral microbiota of aging dogs. The goal of this project was to characterize the longitudinal changes in oral microbiota in aged dogs. Oral swabs were taken from ten senior client-owned dogs on 2-3 occasions spanning 24 months and they underwent whole genome shotgun (WGS) sequencing. Cognitive status was established at each sampling time. A statistically significant increase in alpha diversity for bacterial and fungal species was observed between the first and last study visits. Bacteroidetes and proteobacteria were the most abundant bacterial phyla. Porphyromonas gulae was the most abundant bacterial species (11.6% of total reads). The species Lactobacillus gasseri had a statistically significant increase in relative abundance with age whereas Leptotrichia sp. oral taxon 212 had a statistically significant positive longitudinal association with cognition score. There is an increased fungal and bacterial alpha diversity in aging dogs over time and nearly universal oral dysbiosis. The role of the oral microbiota, particularly Leptotrichia and P. gulae and P. gingivalis, in aging and CCDS warrants further investigation.

Keywords: Alzheimer’s disease; canine cognitive dysfunction syndrome; microbiome; oral.

Grants and funding

This research received no external funding but was funded internally by the Dr. Kady M. Gjessing and Rhanna M. Davidson Distinguished Chair in Gerontology.