Application and Technical Challenges in Design, Cloning, and Transfer of Large DNA

Song Bai; Han Luo; Hanze Tong; Yi Wu

doi:10.3390/bioengineering10121425

Application and Technical Challenges in Design, Cloning, and Transfer of Large DNA

Bioengineering (Basel). 2023 Dec 15;10(12):1425. doi: 10.3390/bioengineering10121425.

Authors

Song Bai^{1

2}, Han Luo^{1

2}, Hanze Tong^{1

2}, Yi Wu^{1

2}

Affiliations

¹ Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
² Frontiers Research Institute for Synthetic Biology, Tianjin University, Tianjin 300072, China.

Abstract

In the field of synthetic biology, rapid advancements in DNA assembly and editing have made it possible to manipulate large DNA, even entire genomes. These advancements have facilitated the introduction of long metabolic pathways, the creation of large-scale disease models, and the design and assembly of synthetic mega-chromosomes. Generally, the introduction of large DNA in host cells encompasses three critical steps: design-cloning-transfer. This review provides a comprehensive overview of the three key steps involved in large DNA transfer to advance the field of synthetic genomics and large DNA engineering.

Keywords: genome engineering; large DNA; synthetic biology; synthetic genomics.

Publication types

Review

Grants and funding

This research received no external funding.