Draft genome sequence of the Saccharomyces cerevisiae Spy Cas9 expressing strain IMX2600, a laboratory and platform strain from the CEN.PK lineage for cell-factory research

Marcel van den Broek; Raul A Ortiz-Merino; Nicole X Bennis; Anna K Wronska; Else-Jasmijn Hassing; Pascale Daran-Lapujade; Jean-Marc G Daran

doi:10.1128/mra.00550-23

Draft genome sequence of the Saccharomyces cerevisiae Spy Cas9 expressing strain IMX2600, a laboratory and platform strain from the CEN.PK lineage for cell-factory research

Microbiol Resour Announc. 2024 Feb 15;13(2):e0055023. doi: 10.1128/mra.00550-23. Epub 2023 Dec 22.

Authors

Marcel van den Broek¹, Raul A Ortiz-Merino¹, Nicole X Bennis¹, Anna K Wronska¹, Else-Jasmijn Hassing¹, Pascale Daran-Lapujade¹, Jean-Marc G Daran¹

Affiliation

¹ Department of Biotechnology, Delft University of Technology, Van der Maasweg, Delft, the Netherlands.

Abstract

The biobased-economy aims to create a circular biotechnology ecosystem to transition from a fossil fuel-based to a sustainable industry based on biomass. For this, new microbial cell-factories are essential. We present the draft genome of the CEN.PK-derived Saccharomyces cerevisiae SpyCas9 expressing strain (IMX2600), that serve as chassis of new cell-factories.

Keywords: CRISPR; Saccharomyces cerevisiae; biotechnology; cell factory; metabolic engineering.

Grants and funding

#PPPS1701/Ministry of economic affairs and climate policy, Netherlands