A pan-cancer analysis of the oncogenic role of Golgi transport 1B in human tumors

Bo Tian; Yanan Pang; Ye Gao; Qianqian Meng; Lei Xin; Chang Sun; Xin Tang; Yilin Wang; Zhaoshen Li; Han Lin; Luowei Wang

doi:10.2478/jtim-2023-0002

A pan-cancer analysis of the oncogenic role of Golgi transport 1B in human tumors

J Transl Int Med. 2023 Dec 20;11(4):433-448. doi: 10.2478/jtim-2023-0002. eCollection 2023 Dec.

Authors

Bo Tian¹, Yanan Pang^{1

2}, Ye Gao¹, Qianqian Meng¹, Lei Xin¹, Chang Sun¹, Xin Tang¹, Yilin Wang³, Zhaoshen Li¹, Han Lin¹, Luowei Wang¹

Affiliations

¹ Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
² Shanghai Institute of Pancreatic Diseases, Shanghai 200433, China.
³ Georgetown Preparatory School, North Bethesda 20852, MD, USA.

Abstract

Background: Owing to the aggressiveness and treatment-refractory nature of cancer, ideal candidates for early diagnosis and treatment are needed. Golgi transport 1B (GOLT1B) has been associated with cellular malignant behaviors and immune responses in colorectal and lung cancer, but a systematic pan-cancer analysis on GOLT1B has not been conducted.

Methods: The expression status and clinical association of GOLT1B in The Cancer Genome Atlas (TCGA) were analyzed. Genetic and methylation alterations in GOLT1B were explored. The relationship between GOLT1B and immune cell infiltration was also investigated. Genes related to GOLT1B expression were selected and analyzed.

Results: GOLT1B was highly expressed in most tumors, and there was a positive correlation between GOLT1B expression and clinical pathological parameters. High expression levels of GOLT1B have been associated with poor prognosis of most cancers. Copy number amplification was the primary type of GOLT1B genetic alterations, which was related to the prognosis of pan-cancer cases. There were different levels of GOLT1B promoter methylation across cancer types. The methylation level of the probe cg07371838 and cg25816357 was closely associated with prognosis in diverse cancers. There was also a positive correlation between GOLT1B genetic alterations and CD4+ T lymphocytes, especially the Th2 subset, as well as between GOLT1B expression and the estimated infiltration value of cancer-associated fibroblasts. Serine/threonine kinase receptor-associated protein (STRAP), integrator complex subunit 13 (INTS13), and ethanolamine kinase 1 (ETNK1) were the most relevant genes for GOLT1B expression, and their interactions with GOLT1B were involved in regulating the transforming growth factor (TGF)-β receptor signaling pathway and epithelial-mesenchymal transition (EMT).

Conclusions: This pan-cancer analysis provided a comprehensive understanding of the oncogenic role of GOLT1B, highlighting a potential mechanism whereby GOLT1B influences the tumor microenvironment, as well as cancer immunotherapy.

Keywords: Golgi transport 1B; bioinformatics; immune infiltration; pan-cancer; prognosis.

Grants and funding

This work was supported by the National Natural Science Foundation of China under Grant (No. 82103268); Shanghai Sailing Program under Grant (No. 21YF1459100); China Postdoctoral Science Foundation under Grant (No. 48067); the start-up funds for postdoctoral in Second Military Medical University; and the start-up Scientific Research Fund of Young Teachers in Changhai Hospital (No. 2019QNB01); Shanghai Science and Technology Innovation Action Program (No. 21Y31900100), and 234 clinical research fund of Changhai hospital (No. 2019YXK006).