OsDWARF10, transcriptionally repressed by OsSPL3, regulates the nutritional metabolism of polished rice

Kang Li; Yan Cheng; Chuanying Fang

doi:10.3389/fpls.2023.1322463

OsDWARF10, transcriptionally repressed by OsSPL3, regulates the nutritional metabolism of polished rice

Front Plant Sci. 2023 Dec 7:14:1322463. doi: 10.3389/fpls.2023.1322463. eCollection 2023.

Authors

Kang Li^#^{1

2}, Yan Cheng^#², Chuanying Fang^{1

2}

Affiliations

¹ Hainan Yazhou Bay Seed Laboratory, Scool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, China.
² School of Tropical Agriculture and Forestry, Hainan University, Haikou, China.

^# Contributed equally.

Abstract

Strigolactone (SL) plays essential roles in plant development and the metabolism of rice leaves. However, the impact of SL on the accumulation of nutritional metabolites in polished rice, as well as the transcription factors directly involved in SL synthesis, remains elusive. In this study, we performed a metabolome analysis on polished rice samples from mutants of an SL biosynthetic gene, OsDWARF10 (OsD10). Compared with those in the wild type plants, primary and secondary metabolites exhibited a series of alterations in the d10 mutants. Notably, the d10 mutants showed a substantial increase in the amino acids and vitamins content. Through a yeast one-hybridization screening assay, we identified OsSPL3 as a transcription factor that binds to the OsD10 promoter, thereby inhibiting OsD10 transcription in vivo and in vitro. Furthermore, we conducted a metabolic profiling analysis in polished rice from plants that overexpressed OsSPL3 and observed enhanced levels of amino acids and vitamins. This study identified a novel transcriptional repressor of the SL biosynthetic gene and elucidated the regulatory roles of OsSPL3 and OsD10 on the accumulation of nutritional metabolites in polished rice.

Keywords: OsD10; OsSPL3; metabolome; polished rice; strigolactones; transcriptional regulation.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the National Natural Science Foundation of China (NO. 32300452), the Natural Science Foundation of Hainan Province (NO. 321RC463), and the National Natural Science Foundation of China (NO. 31960063).