Oxygen interaction with the carbon surface is one of the most important topics of study in the field of material chemistry. In this work, experimental evidence for molecular oxygen dissociative chemisorption on a carbon surface at room temperature is shown for the first time. It was determined that the process occurs only on the bare carbon surface, and the quantitative description of the phenomena is possible using the Temkin model, which explains an almost linear decrease in the calorimetric heat of adsorption. The results provided by in situ infrared studies show that surface carbonyl oxides appear as intermediates of final functionality, i.e., carbonyl structures. Examining the thermal stability of surface structures shows that all surface species decompose at temperatures below 500 °C, leaving a pristine carbon surface.