Characteristics and expression of lncRNA and transposable elements in Drosophila aneuploidy

Shuai Zhang; Ruixue Wang; Xilin Zhu; Ludan Zhang; Xinyu Liu; Lin Sun

doi:10.1016/j.isci.2023.108494

Characteristics and expression of lncRNA and transposable elements in Drosophila aneuploidy

iScience. 2023 Nov 19;26(12):108494. doi: 10.1016/j.isci.2023.108494. eCollection 2023 Dec 15.

Authors

Shuai Zhang¹, Ruixue Wang¹, Xilin Zhu¹, Ludan Zhang¹, Xinyu Liu¹, Lin Sun^{1

2}

Affiliations

¹ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
² Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.

Abstract

Aneuploidy can globally affect the expression of the whole genome, which is detrimental to organisms. Dosage-sensitive regulators usually have multiple intermolecular interactions, and changes in their stoichiometry are responsible for the dysregulation of the regulatory network. Currently, studies on noncoding genes in aneuploidy are relatively rare. We studied the characteristics and expression profiles of long noncoding RNAs (lncRNAs) and transposable elements (TEs) in aneuploid Drosophila. It is found that lncRNAs and TEs are affected by genomic imbalance and appear to be more sensitive to an inverse dosage effect than mRNAs. Several dosage-sensitive lncRNAs and TEs were detected for their expression patterns during embryogenesis, and their biological functions in the ovary and testes were investigated using tissue-specific RNAi. This study advances our understanding of the noncoding sequences in imbalanced genomes and provides a novel perspective for the study of aneuploidy-related human diseases such as cancer.

Keywords: Expression study; Genomic analysis; Model organism; Transcriptomics.