Many genetic pathways and environmental factors have been shown to affect Drosophila melanogaster adult body size. Larval density often varies considerably between vials, even when the same number of females of the same genotype are allowed to lay eggs in the vials for the same amount of time. To more accurately quantify the effects that larval population density has on pupal size, we established cultures of 1, 2, 10, 25, 50, 75 or 100 first instar larvae into vials and measured pupal length. We collected Oregon-R eggs on apple juice plates in six different cages and generated replicate cultures. We found that pupal size decreases as larval density in the culture increases by 25 individuals. The difference between male and female length remained relatively constant at each density (0.2 mm), but overall size decreased. The mean size differences between vials with 1 larvae and 100 larvae is 0.1(+/-0.02) mm in females and 0.11(+/-0.02) mm in males. These results suggest that fecundity and sex ratio could complicate results in Drosophila size studies.
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