Transcriptome analysis during axillary bud growth in chrysanthemum (chrysanthemum× morifolium)

Yijun Chen; Qin Ling; Xin Li; Qiqi Ma; ShaoKang Tang; Pan Yuanzhi; Qing-Lin Liu; Yin Jia; Xue Yong; Beibei Jiang

doi:10.7717/peerj.16436

Transcriptome analysis during axillary bud growth in chrysanthemum (chrysanthemum× morifolium)

PeerJ. 2023 Dec 15:11:e16436. doi: 10.7717/peerj.16436. eCollection 2023.

Authors

Yijun Chen^#¹, Qin Ling^#¹, Xin Li¹, Qiqi Ma¹, ShaoKang Tang¹, Pan Yuanzhi¹, Qing-Lin Liu¹, Yin Jia¹, Xue Yong¹, Beibei Jiang¹

Affiliation

¹ College of Landscape Architecture, Sichuan Agricultural University, Chendu, Sichuan, China.

^# Contributed equally.

Abstract

The chrysanthemum DgLsL gene, homologous with tomato Ls, is one of the earliest expressed genes controlling axillary meristem initiation. In this study, the wild-type chrysanthemum (CW) and DgLsL-overexpressed line 15 (C15) were used to investigate the regulatory mechanism of axillary bud development in chrysanthemum. Transcriptome sequencing was carried out to detect the differentially expressed genes of the axillary buds 0 h, 24 h and 48 h after decapitation. The phenotypic results showed that the number of axillary buds of C15 was significantly higher than CW. A total of 9,224 DEGs were identified in C15-0 vs. CW-0, 10,622 DEGs in C15-24 vs. CW-24, and 8,929 DEGs in C15-48 vs. CW-48.GO and KEGG pathway enrichment analyses showed that the genes of the flavonoid, phenylpropanoids and plant hormone pathways appeared to be differentially expressed, indicating their important roles in axillary bud germination. DgLsL reduces GA content in axillary buds by promoting GA2ox expression.These results confirmed previous studies on axillary bud germination and growth, and revealed the important roles of genes involved in plant hormone biosynthesis and signal transduction, aiding in the study of the gene patterns involved in axillary bud germination and growth.

Keywords: Axillary bud; Chrysanthemum morifolium; DgLsL; Differentially expressed genes; Transcriptome.

MeSH terms

Cell Division
Chrysanthemum* / genetics
Gene Expression Profiling / methods
Plant Growth Regulators* / genetics

Substances

Plant Growth Regulators

Grants and funding

This research was supported by the National Natural Science Foundation of China grant number 31800601 and Innovation Training Program for College Students of Sichuan Agricultural University grant number 040-2021998185. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.