Impact of light spectra on photo-fermentative biohydrogen production by Rhodobacter sphaeroides KKU-PS1

Ming Foong Tiang; Muhammad Alif Fitri Hanipa; Safa Senan Mahmod; Muhammad Tarmidzi Zainuddin; Abdullah Amru Indera Lutfi; Jamaliah Md Jahim; Mohd Sobri Takriff; Alissara Reungsang; Shu-Yii Wu; Peer Mohamed Abdul

doi:10.1016/j.biortech.2023.130222

Impact of light spectra on photo-fermentative biohydrogen production by Rhodobacter sphaeroides KKU-PS1

Bioresour Technol. 2024 Feb:394:130222. doi: 10.1016/j.biortech.2023.130222. Epub 2023 Dec 17.

Affiliations

¹ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia.
² Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia; Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis, UniMAP, 02600 Arau, Perlis, Malaysia; Centre of Excellence for Biomass Utilization, Universiti Malaysia Perlis, UniMAP, 02600 Arau, Perlis, Malaysia.
³ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bandar Baru Bangi, Selangor Darul Ehsan, Malaysia.
⁴ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bandar Baru Bangi, Selangor Darul Ehsan, Malaysia; Department of Mechanical and Nuclear Engineering, College of Engineering, University of Sharjah, United Arab Emirates.
⁵ Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
⁶ Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan; Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan.
⁷ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia; Centre of Excellence for Biomass Utilization, Universiti Malaysia Perlis, UniMAP, 02600 Arau, Perlis, Malaysia; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bandar Baru Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: peer@ukm.edu.my.

PMID: 38109981
DOI: 10.1016/j.biortech.2023.130222

Abstract

Purple non-sulphur bacteria can only capture up to 10 % light spectra and only 1-5 % of light is converted efficiently for biohydrogen production. To enhance light capture and conversion efficiencies, it is necessary to understand the impact of various light spectra on light harvesting pigments. During photo-fermentation, Rhodobacter sphaeroides KKU-PS1 cultivated at 30 °C and 150 rpm under different light spectra has been investigated. Results revealed that red light is more beneficial for biomass accumulation, whereas green light showed the greatest impact on photo-fermentative biohydrogen production. Light conversion efficiency by green light is 2-folds of that under control white light, hence photo-hydrogen productivity is ranked as green > red > orange > violet > blue > yellow. These experimental data demonstrated that green and red lights are essential for photo-hydrogen and biomass productions of R. sphaeroides and a clearer understanding that possibly pave the way for further photosynthetic enhancement research.

Keywords: Light conversion efficiency; Light wavelength; Photo-fermentation; Photo-hydrogen; Rhodobacter sphaeroides.

MeSH terms

Fermentation
Green Light
Hydrogen
Light
Rhodobacter sphaeroides*

Substances

Hydrogen