p16 and p53 can Serve as Prognostic Markers for Head and Neck Squamous Cell Carcinoma

Yue Wang; Fang Wang; Shuhan Wang; Lingnan Zhang; Honghai Fu; Legang Sun; Wenlong Wang; Chunxia Liu; Wenhao Ren; Ling Gao; Guoyi Xing; Xiangrui Ma

doi:10.1016/j.identj.2023.11.007

p16 and p53 can Serve as Prognostic Markers for Head and Neck Squamous Cell Carcinoma

Int Dent J. 2023 Dec 16:S0020-6539(23)00971-1. doi: 10.1016/j.identj.2023.11.007. Online ahead of print.

Authors

Yue Wang¹, Fang Wang², Shuhan Wang³, Lingnan Zhang⁴, Honghai Fu², Legang Sun², Wenlong Wang⁵, Chunxia Liu⁵, Wenhao Ren⁶, Ling Gao⁷, Guoyi Xing⁸, Xiangrui Ma⁹

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China; Department of stomatology, ZiBo Central Hospital, ZiBo, Shandong, China; School of Stomatology, Binzhou Medical University, Yantai, China.
² Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China; School of Stomatology, Binzhou Medical University, Yantai, China.
³ School of Stomatology, Qilu Medical University, ZiBo, Shangdong, China.
⁴ School of Stomatology, Binzhou Medical University, Yantai, China; Department of Orthodontics, Binzhou Medical University Hospital, Binzhou, Shandong, China.
⁵ Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China.
⁶ Department of Oral and Maxillofacial Reconstruction, the Affiliated Hospital of Qingdao University, Qingdao, China.
⁷ Department of Orthodontics, Binzhou Medical University Hospital, Binzhou, Shandong, China; Key Lab of Oral Clinical Medicine, the Affiliated Hospital of Qingdao University, Qingdao, China.
⁸ Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China; School of Stomatology, Binzhou Medical University, Yantai, China; Wuhan Dongxihu District People's Hospital.
⁹ Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China. Electronic address: wxy851225@163.com.

PMID: 38105167
DOI: 10.1016/j.identj.2023.11.007

Abstract

Objective: The present study aimed to explore the expression and clinical significance of human papilloma virus-related pathogenic factors (p16, cyclin D1, p53) in patients with head and neck squamous cell carcinoma (HNSCC) and construct a predictive model.

Methods: The Cancer Genome Atlas was used to obtain clinical data for 112 patients with HNSCC. Expression of p16, p53, and cyclin D1 was quantified. We used the survival package of the R program to set the cut-off value. Values above the cut-off were considered positive, while values below the cut-off were negative. Kaplan-Meier analysis and univariate and multivariate Cox regression analyses were performed to investigate prognostic clinicopathological indicators and the expression of p16, p53, and cyclin D1. A predictive model was constructed based on the results of multifactor Cox regression analysis, and the accuracy of the predictive model was verified through final calibration analysis. Follow-up of patients with HNSCC at the Affiliated Hospital of Binzhou Medical University was conducted from 2015 to 2017, and reliability of the predictive model was validated based on follow-up data and molecular expression levels.

Results: According to the results, expression of p16 and p53 was significantly associated with prognosis (P < .05). The predictive model constructed based on the expression levels of p16 and p53 was useful for evaluating the prognosis of patients with HNSCC. The predictive model was validated using follow-up data obtained from the hospital, and the trend of the follow-up results was consistent with the predictive model.

Conclusion: p16 and p53 can be used as key indicators to predict the prognosis of HNSCC patients and as critical immunohistochemical indicators in clinical practice. The survival model constructed based on p16 and p53 expression levels reliably predicts patient prognosis.

Keywords: Head and neck squamous cell carcinoma; Nomogram; p16; p53.