DNA Vaccine Encoding the Artificial T-Cell Polyepitope Immunogen of Tick-Borne Encephalitis Virus

D N Kisakov; D V Antonets; E V Shaburova; L A Kisakova; E V Tigeeva; V A Yakovlev; E V Starostina; M B Borgoyakova; E V Protopopova; V A Svyatchenko; V B Loktev; A P Rudometov; A A Ilyichev; T S Nepomnyashchikh; L I Karpenko

doi:10.1007/s10517-023-05970-4

DNA Vaccine Encoding the Artificial T-Cell Polyepitope Immunogen of Tick-Borne Encephalitis Virus

Bull Exp Biol Med. 2023 Nov;176(1):72-76. doi: 10.1007/s10517-023-05970-4. Epub 2023 Dec 13.

Authors

Affiliations

¹ State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. def_2003@mail.ru.
² State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.

PMID: 38091143
DOI: 10.1007/s10517-023-05970-4

Abstract

A promising approach to the development of new means for preventing infection caused by tick-borne encephalitis virus can be DNA vaccines encoding polyepitope T-cell immunogens. A DNA vaccine pVAX-AG4-ub encoding an artificial polyepitope immunogen that includes cytotoxic and T-helper epitopes from the NS1, NS3, NS5, and E proteins of the tick-borne encephalitis virus has been obtained. The developed construct ensured the synthesis of the corresponding mRNAs in transfected eukaryotic cells. Immunization of mice with pVAX-AG4-ub induced the formation of a virus-specific T-cell response providing 50% protection from lethal infection with the virus.

Keywords: DNA vaccines; artificial T-cell immunogen; tick-borne encephalitis.

MeSH terms

Animals
Encephalitis Viruses, Tick-Borne* / genetics
Immunization
Mice
T-Lymphocytes
Vaccines, DNA* / genetics
Viral Vaccines* / genetics

Substances

Vaccines, DNA
Viral Vaccines