The utilization of single-cell sequencing technology in investigating the immune microenvironment of ccRCC

Front Immunol. 2023 Nov 27:14:1276658. doi: 10.3389/fimmu.2023.1276658. eCollection 2023.

Abstract

The growth and advancement of ccRCC are strongly associated with the presence of immune infiltration and the tumor microenvironment, comprising tumor cells, immune cells, stromal cells, vascular cells, myeloid-derived cells, and extracellular matrix (ECM). Nevertheless, as a result of the diverse and constantly evolving characteristics of the tumor microenvironment, prior advanced sequencing methods have frequently disregarded specific less prevalent cellular traits at varying intervals, thereby concealing their significance. The advancement and widespread use of single-cell sequencing technology enable us to comprehend the source of individual tumor cells and the characteristics of a greater number of individual cells. This, in turn, minimizes the impact of intercellular heterogeneity and temporal heterogeneity of the same cell on experimental outcomes. This review examines the attributes of the tumor microenvironment in ccRCC and provides an overview of the progress made in single-cell sequencing technology and its particular uses in the current focus of immune infiltration in ccRCC.

Keywords: ScRNA-seq; T-cell; TME; ccRCC; time.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Renal Cell*
  • Extracellular Matrix
  • Humans
  • Kidney Neoplasms*
  • Myeloid Cells
  • Technology
  • Tumor Microenvironment

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project is supported by the Liaoning province Doctoral Research Startu p Fund Program(NO.2021-BS-209).