Comparative genomics analysis of Stenotrophomonas maltophilia strains from a community

Yini Li; Xin Liu; Lingzhi Chen; Xiao Shen; Haihong Wang; Ruiyu Guo; Xiang Li; Zehui Yu; Xiaoli Zhang; Yingshun Zhou; Li Fu

doi:10.3389/fcimb.2023.1266295

Comparative genomics analysis of Stenotrophomonas maltophilia strains from a community

Front Cell Infect Microbiol. 2023 Nov 28:13:1266295. doi: 10.3389/fcimb.2023.1266295. eCollection 2023.

Authors

Yini Li¹, Xin Liu², Lingzhi Chen¹, Xiao Shen², Haihong Wang², Ruiyu Guo², Xiang Li², Zehui Yu³, Xiaoli Zhang⁴, Yingshun Zhou², Li Fu⁵

Affiliations

¹ Department of Ultrasound, Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou, China.
² Department of Pathogen Biology, School of Basic Medicine, Public Center of Experimental Technology of Pathogen Biology Technology Platform, Southwest Medical University, Luzhou, China.
³ Laboratory Animal Center, Southwest Medical University, Luzhou, China.
⁴ Department of Allergy, Jiangnan University Medical Center, Wuxi, China.
⁵ Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou, China.

Abstract

Background: Stenotrophomonas maltophilia is a multidrug-resistant (MDR) opportunistic pathogen with high resistance to most clinically used antimicrobials. The dissemination of MDR S. maltophilia and difficult treatment of its infection in clinical settings are global issues.

Methods: To provide more genetic information on S. maltophilia and find a better treatment strategy, we isolated five S. maltophilia, SMYN41-SMYN45, from a Chinese community that were subjected to antibiotic susceptibility testing, biofilm formation assay, and whole-genome sequencing. Whole-genome sequences were compared with other thirty-seven S. maltophilia sequences.

Results: The five S. maltophilia strains had similar antibiotic resistance profiles and were resistant to β-lactams, aminoglycosides, and macrolides. They showed similar antimicrobial resistance (AMR) genes, including various efflux pumps, β-lactamase resistance genes (blaL1/2), aminoglycoside resistance genes [aac(6'), aph(3'/6)], and macrolide-resistant gene (MacB). Genome sequencing analysis revealed that SMYN41-SMYN45 belonged to sequence type 925 (ST925), ST926, ST926, ST31, and ST928, respectively, and three new STs were identified (ST925, ST926, and ST928).

Conclusion: This study provides genetic information by comparing genome sequences of several S. maltophilia isolates from a community of various origins, with the aim of optimizing empirical antibiotic medication and contributing to worldwide efforts to tackle antibiotic resistance.

Keywords: Stenotrophomonas maltophilia; biofilm; genome analysis; multidrug resistance; whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Infective Agents* / pharmacology
Drug Resistance, Microbial
Genomics
Gram-Negative Bacterial Infections*
Humans
Microbial Sensitivity Tests
Stenotrophomonas maltophilia* / genetics

Substances

Anti-Bacterial Agents
Anti-Infective Agents

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the Joint Project of Southwest Medical University [2021SNXNYD01, 2021NJXNYD06], Sichuan Province Science and Technology Project [2022YFS0631 and 2022YFS0632], Natural Science Foundation of Luzhou (2021-NYF-20), and Southwest Medical University Foundation (2021ZKZD002).