Snake venom cysteine-rich secretory protein from Mojave rattlesnake venom (Css-CRiSP) induces acute inflammatory responses on different experimental models

Emelyn Salazar; Abcde Cirilo; Armando Reyes; Martha Barrientos; Jacob Galan; Elda E Sánchez; Montamas Suntravat

doi:10.1016/j.toxcx.2023.100180

Snake venom cysteine-rich secretory protein from Mojave rattlesnake venom (Css-CRiSP) induces acute inflammatory responses on different experimental models

Toxicon X. 2023 Dec 7:21:100180. doi: 10.1016/j.toxcx.2023.100180. eCollection 2024 Mar.

Authors

Emelyn Salazar¹, Abcde Cirilo¹, Armando Reyes¹, Martha Barrientos¹, Jacob Galan², Elda E Sánchez^{1

3}, Montamas Suntravat^{1

3}

Affiliations

¹ National Natural Toxins Research Center, Texas A&M University-Kingsville, Kingsville, TX, USA.
² Department of Human Genetics, University of Texas Rio Grande Valley, Brownsville, TX, USA.
³ Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX, USA.

Abstract

Snake venoms contain various molecules known for activating innate immunity and causing local effects associated with increased vascular permeability, such as vascular leakage and edema, common symptoms seen in snakebite envenomings. We have demonstrated that snake venom cysteine-rich secretory proteins (svCRiSPs) from North American pit vipers increase vascular permeability. This study aimed to explore the functional role of CRiSP isolated from Mojave rattlesnake (Crotalus scutulatus scutulatus) venom (Css-CRiSP) on the activation of inflammatory responses in different models. We measured the release of inflammatory mediators in cultured human dermal blood endothelial cells (HDBEC), lymphatic endothelial cells (HDLEC) and monocyte-derived macrophages (MDM) at 0.5, 1, 3, 6, and 24 h after treatment with Css-CRiSP (1 μM). We also determined the acute inflammatory response in BALB/c mice 30 min after intraperitoneal injection of the toxin (2 μg/mouse). Css-CRiSP induced the production of IL-8 and IL-6, but not TNF-α, in HDBEC and HDLEC in a time-dependent manner. In addition, Css-CRiSP significantly enhanced the production of IL-6, TNF-α, IL-8, and IL-1β in MDM. Moreover, it caused a remarkable increase of chemotactic mediators in the exudates of experimental mice. Our results reveal that Css-CRiSPs can promote a sustained release of inflammatory mediators on cell lines and an acute activation of innate immunity in a murine model. These findings contribute to the growing body of evidence supporting the involvement of svCRiSPs in the augmentation of envenomation effects, specifically, the role of svCRiSPs in inducing vascular dysfunction, initiating early inflammatory responses, and facilitating the activation of leukocytes and releasing mediators. These findings will lead to a better understanding of the pathophysiology of envenoming by Mojave rattlesnakes, allowing the development of more efficient therapeutic strategies.

Keywords: Cytokine; Endothelial cells; Inflammatory response; Mojave rattlesnake (Crotalus scutulatus scutulatus); Monocyte-derived macrophages (MDM); Snake venom cysteine-rich secretory protein (svCRiSP).