Enhancing docosahexaenoic acid production in Aurantiochytrium species using atmospheric and room temperature plasma mutagenesis and comprehensive multi-omics analysis

Qing Wang; Wenbiao Jin; Xu Zhou; Chuan Chen; Wei Han; T M Indra Mahlia; Xuan Li; Guangming Jiang; Huan Liu; Qilin Wang

doi:10.1016/j.scitotenv.2023.169217

Enhancing docosahexaenoic acid production in Aurantiochytrium species using atmospheric and room temperature plasma mutagenesis and comprehensive multi-omics analysis

Sci Total Environ. 2024 Feb 20:912:169217. doi: 10.1016/j.scitotenv.2023.169217. Epub 2023 Dec 10.

Authors

Qing Wang¹, Wenbiao Jin², Xu Zhou³, Chuan Chen⁴, Wei Han¹, T M Indra Mahlia⁵, Xuan Li⁵, Guangming Jiang⁶, Huan Liu⁵, Qilin Wang⁵

Affiliations

¹ State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China.
² State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China. Electronic address: jinwb@hit.edu.cn.
³ State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China. Electronic address: zhouxu@hit.edu.cn.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province 150090, China.
⁵ Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia.
⁶ School of Civil, Mining and Environmental Engineering, University of Wollongong, NSW 2522 Wollongong, Australia.

PMID: 38081429
DOI: 10.1016/j.scitotenv.2023.169217

Abstract

Aurantiochytrium sp. belongs to marine heterotrophic single-cell protist, which is an important decomposer in marine ecosystem. Aurantiochytrium sp. has gained notoriety because of its ability to accumulate high-value docosahexaenoic acid (DHA), but the key factors of DHA synthesis were unclear at present. In this study, Atmospheric and Room Temperature Plasma technology was applied to the mutagenic breeding of Aurantiochytrium sp., and transcriptomics and proteomics were adopted to analyze the DHA-biosynthesis mechanism. According to the growth and DHA accumulation profiles, the mutant strain Aurantiochytrium sp. R2A35 was selected. The DHA content in total lipids was greatly improved from 49.39 % of the wild strain R2 to 63.69 % of the mutant strain. Moreover, the DHA content in the biomass of Aurantiochytrium sp. R2A35 as 39.72 % was the highest DHA productivity reported so far. The differentially expressed genes distinguished from transcriptome and the TMT-identified differential proteins distinguished from proteome confirmed that the expression of acetyl-CoA carboxylase and ketoacyl reductase was up-regulated by 4.78-fold and 6.95-fold, respectively and the fatty acid synthase was concurrently down-regulated by 2.79-fold, so that more precursor was transported to the polyketide synthase pathway, thereby increasing the DHA yield in Aurantiochytrium sp. R2A35. This research would provide reference for the DHA metabolism process and contribute to the understanding of the decomposer - Aurantiochytrium sp. in marine ecosystems.

Keywords: Aurantiochytrium sp.; Differentially expressed gene; Docosahexaenoic acid; Mutagenesis; TMT-identified differential protein.

MeSH terms

Docosahexaenoic Acids* / metabolism
Ecosystem
Multiomics
Mutagenesis
Stramenopiles* / metabolism
Temperature

Substances

Docosahexaenoic Acids