DNAzyme Nanomachine with Fluorogenic Substrate Delivery Function: Advancing Sensitivity in Nucleic Acid Detection

Zain Hussein; Moustapha A Y Nour; Anastasia V Kozlova; Dmitry M Kolpashchikov; Andrey B Komissarov; Ahmed A El-Deeb

doi:10.1021/acs.analchem.3c04420

DNAzyme Nanomachine with Fluorogenic Substrate Delivery Function: Advancing Sensitivity in Nucleic Acid Detection

Anal Chem. 2023 Dec 26;95(51):18667-18672. doi: 10.1021/acs.analchem.3c04420. Epub 2023 Dec 11.

Authors

Zain Hussein^{1

2}, Moustapha A Y Nour^{1

2}, Anastasia V Kozlova^{1

2}, Dmitry M Kolpashchikov^{3

4

5}, Andrey B Komissarov⁶, Ahmed A El-Deeb^{1

2}

Affiliations

¹ Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, Lomonosova 9, Saint Petersburg, 191002, Russian Federation.
² Advanced Engineering School, 423450 Almetyevsk, Russian Federation.
³ Chemistry Department, University of Central Florida, 4000 Central Florida Blvd., Orlando, Florida 32816, United States.
⁴ Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida 32816, United States.
⁵ National Center for Forensic Science, University of Central Florida, Orlando, Florida 32816, United States.
⁶ Smorodintsev Research Institute of Influenza, 197376 Saint Petersburg, Russian Federation.

PMID: 38079240
DOI: 10.1021/acs.analchem.3c04420

Abstract

We have developed a hook-equipped DNA nanomachine (HDNM) for the rapid detection of specific nucleic acid sequences without a preamplification step. HDNM efficiently unwinds RNA structures and improves the detection sensitivity. Compared to the hookless system, HDNM offers an 80-fold and 13-fold enhancement in DNA and RNA detection, respectively, reducing incubation time from 3 to 1 h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Biosensing Techniques*
DNA / chemistry
DNA, Catalytic* / chemistry
Fluorescent Dyes / chemistry
RNA

Substances

DNA, Catalytic
Fluorescent Dyes
DNA
RNA