Spermine induced endogenous signal ameliorates osmotic stress in Lens culinaris

Nilanjan Chakraborty; Anik Sarkar; Krishnendu Acharya

doi:10.1007/s12298-023-01374-w

Spermine induced endogenous signal ameliorates osmotic stress in Lens culinaris

Physiol Mol Biol Plants. 2023 Oct;29(10):1591-1603. doi: 10.1007/s12298-023-01374-w. Epub 2023 Oct 28.

Authors

Nilanjan Chakraborty¹, Anik Sarkar^{2

3}, Krishnendu Acharya²

Affiliations

¹ Department of Botany, Scottish Church College, Kolkata, West Bengal 700006 India.
² Molecular and Applied Mycology and Plant Pathology Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, West Bengal 700019 India.
³ Department of Science, St. Xavier's College Jaipur, Nevta-Mahapura Road, Jaipur, 302029 India.

PMID: 38076773
PMCID: PMC10709289 (available on 2024-10-01)
DOI: 10.1007/s12298-023-01374-w

Abstract

The present study evaluates the alleviation of polyethylene glycol (PEG) mediated osmotic stress by exogenous spermine (SP) application on seeds of Lens culinaris. Seeds were soaked overnight with SP (0.05 M) and PEG (10%) separately. Third set was prepared by combined treatment with SP (0.05 M) and PEG (10%). Further, various physiological parameters, defense enzymes, phenol, flavonoid, nitric oxide (NO), proline content, chromosomal abnormality studies etc. was conducted after 5 days of incubation. PEG application significantly hampered the seed germination, root length, shoot length, relative water content and vigour index. Application of PEG induced electrolytic leakage in root cells. PEG application also significantly reduced the activities of different defense parameters viz. peroxidase, polyphenol oxidase, phenylalanine ammonia lyase and β-1,3 glucanase, total phenol and flavonoid accumulation over control. Reactive oxygen species, lipid peroxidation, cell death was found significantly high in PEG treated sets. However, NO level has been significantly decreased by PEG treatment. Cytological studies showed reduced mitotic index and highest abnormalities under osmotic stress. Contrary to that, application of SP either alone or in combination with PEG showed improved responses of aforesaid defense parameters compared to control and solely PEG treated sets. Interestingly, cytological abnormalities were also reduced in SP treated roots. Overall, these results suggests that SP application on seeds reduced the PEG induced damage and intricately improved the defense mechanisms which might help to fight against oxidative stress.

Keywords: Cell death; Defense enzymes; Nitric oxide; Reactive oxygen species; Spermine.

© Prof. H.S. Srivastava Foundation for Science and Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.