Knockdown of FOXRED2 restrains proliferation, invasion and migration of human melanoma cells

Mujin Li; Si Qin; Zhijun Xu; Yingjun Feng; Sihui Li; Xianwen Li; Zhenyu Lu; Shuting Huang; Siman Shi; Yixue Duan; Ju Wen

doi:10.1016/j.heliyon.2023.e22588

Knockdown of FOXRED2 restrains proliferation, invasion and migration of human melanoma cells

Heliyon. 2023 Nov 30;9(12):e22588. doi: 10.1016/j.heliyon.2023.e22588. eCollection 2023 Dec.

Authors

Mujin Li^{1

2}, Si Qin¹, Zhijun Xu², Yingjun Feng¹, Sihui Li¹, Xianwen Li¹, Zhenyu Lu³, Shuting Huang¹, Siman Shi¹, Yixue Duan¹, Ju Wen¹

Affiliations

¹ Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China.
² Shenshan Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Shanwei, 516621, China.
³ Department of Dermatology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510799, China.

Abstract

Objectives: This experiment investigated the role of the FAD-dependent oxidoreductase domain-containing 2 (FOXRED2) in the development of cutaneous malignant melanoma.

Methods: We explored the expression and prognostic effects of FOXRED2 in cutaneous malignant melanoma by performing bioinformatics analyses and immunohistochemical staining experiments and verified the biological influence of FOXRED2 on human melanoma cells using in vitro experiments.

Results: FOXRED2 expression was significantly higher in cutaneous malignant melanoma compared to normal skin and nevus tissues and closely associated with prognosis. The expression levels of FOXRED2 mRNA and protein were significantly upregulated in human melanoma cell lines, and knocking down FOXRED2 expression inhibits proliferation, invasion, and migration, promotes apoptosis, and alters tumor cell biology in A2058 and A375 cells.

Conclusion: FOXRED2 may play a crucial role in the development and progression of cutaneous malignant melanoma.

Keywords: Bioinformatics; Cutaneous malignant melanoma; FOXRED2 protein; Human melanoma cell lines; Tumor biology.