Rapid Raman spectroscopic identification of three homoisoflavanones in polygonatum odoratum based on twice chromatography derivatization

Tao Xu; Qian Li; Feng Xu; Li Li; Shuang Li; Yanli Dong; Xin Liang; Hongguang Zhang; Peng Hou; Ge Sun; Meng Cao; Hao Dou

doi:10.1016/j.heliyon.2023.e22441

Rapid Raman spectroscopic identification of three homoisoflavanones in polygonatum odoratum based on twice chromatography derivatization

Heliyon. 2023 Nov 20;9(12):e22441. doi: 10.1016/j.heliyon.2023.e22441. eCollection 2023 Dec.

Authors

Tao Xu¹, Qian Li¹, Feng Xu¹, Li Li¹, Shuang Li¹, Yanli Dong², Xin Liang¹, Hongguang Zhang¹, Peng Hou¹, Ge Sun¹, Meng Cao¹, Hao Dou¹

Affiliations

¹ School of Pharmacy, Qiqihar Medical University, Qiqihar, China.
² Qiqihar Drug Inspection and Testing Center, Qiqihar, 161006, China.

Abstract

The aim of this study was to establish a new method for the determination of homoisoflavanones (Ⅲ, Ⅳ, V) in polygonatum odoratum(POD) by combination of thin layer chromatography (TLC) and chemical derivative resonance Raman spectroscopy (RRS). The twice chromatography method of TLC was used to improve the specificity of the component to be tested; the method of the relative Rf was used to reduce the use of the reference substance of the component to be tested; the chemical derivatization method was used to improve the signal intensity of Raman spectrum for the component to be tested in POD, so as to obtain a trace amount fingerprint structure information of the measured component. The method exhibits robust specificity, high sensitivity, and reliable stability, there by offering a novel reference approach for the identification and evaluation of homoisoflavanones (Ⅲ, Ⅳ, V) in POD.

Keywords: Homoisoflavanones; Polygonatum odoratum; Relative Rf; Resonance Raman spectrum（RRS）; Twice chromatography.