Complete genome sequence data of chitin-degrading Bacillus velezensis RB.IBE29

Dinh Minh Tran; Thi Huyen Nguyen; Anh Dzung Nguyen

doi:10.1016/j.dib.2023.109815

Complete genome sequence data of chitin-degrading Bacillus velezensis RB.IBE29

Data Brief. 2023 Nov 17:51:109815. doi: 10.1016/j.dib.2023.109815. eCollection 2023 Dec.

Authors

Dinh Minh Tran¹, Thi Huyen Nguyen¹, Anh Dzung Nguyen¹

Affiliation

¹ Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam.

Abstract

This work reports the complete genome sequence of chitinolytic Bacillus velezensis RB.IBE29 recently isolated from the rhizosphere of black pepper cultivated in the Central Highlands region of Vietnam. This bacterium had strong antagonistic activity against phytopathogens and possessed a novel chitinase system. The complete genome of strain RB.IBE29 was sequenced using the platforms of Illumina (2×150 PE) and Oxford Nanopore technologies. Assembly showed that strain RB.IBE29 has one 3,957,092-bp circular chromosome with 46.5 % G+C content. DFAST analysis revealed the genome contains 3819 protein-coding genes, 27 rRNAs, 86 tRNAs, 1 tmRNA, 144 pseudogenes, and shares an ANI value of 97.51 % with that of reported B. velezensis NRRL B-41580. The B. velezensis RB.IBE29 genome possesses at least 42 genes concerning heavy metal resistance and plant-growth promotion. CAZymes analysis showed that 103 genes coding for carbohydrate-active enzymes were predicted in the genome, including 41 genes for glycoside hydrolases, 34 genes for glycosyl transferases, 3 genes for polysaccharide lyases, 17 genes for carbohydrate esterases, 6 genes for auxiliary activities, and 2 genes for carbohydrate-binding modules. Of these deduced enzymes, at least 8 probably possess activities against phytopathogens, such as family 18 chitinases, family 16 glucanase, and family 46 chitosanase. AntiSMASH analysis exhibited that 15 biosynthetic gene clusters were found in the genome; among them, 5 show no sequence similarity to known bacterial clusters. The raw sequences in this work were deposited in Mendeley Data. The complete genome sequence of strain RB.IBE29 was submitted to the DDBJ/GenBank/EMBL under accession number AP028932. The obtained data provide insight into the biocontrol ability and plant-growth promotion of B. velezensis RB.IBE29. The data are valuable for further explorations concerning crop production and other fields using gene expression approaches.

Keywords: Bacillus velezensis; Complete genome sequence; Genes involved in antifungal activity; Genes involved in plant growth promotion.