ADSC-derived exosomes-coupled decellularized matrix for endometrial regeneration and fertility restoration

Xiaoying Jin; Yangyang Dai; Liaobing Xin; Zi Ye; Jiayu Chen; Qianhong He; Xin Chen; Xiaodong Xu; Guanghui Song; Xiaohua Yu; Songying Zhang

doi:10.1016/j.mtbio.2023.100857

ADSC-derived exosomes-coupled decellularized matrix for endometrial regeneration and fertility restoration

Mater Today Bio. 2023 Nov 15:23:100857. doi: 10.1016/j.mtbio.2023.100857. eCollection 2023 Dec.

Authors

Xiaoying Jin^{1

2

3}, Yangyang Dai^{1

2

3}, Liaobing Xin^{1

2

3}, Zi Ye^{1

2

3}, Jiayu Chen⁴, Qianhong He⁴, Xin Chen⁴, Xiaodong Xu⁴, Guanghui Song^{1

2

3}, Xiaohua Yu⁴, Songying Zhang^{1

2

3}

Affiliations

¹ Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016, Hangzhou, China.
² Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China.
³ Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, China.
⁴ Hangzhou Philosopher's Stone Biotechnology Co., Ltd., Hangzhou, Zhejiang, PR China.

Abstract

Endometrium is suspectable to severe injury due to recurrent abortion, curettage or intrauterine infection which could lead to pathological conditions and sabotage women's fertility. Promoting endometrium regeneration is the core of the treatments to uterine related infertility. Patients who received traditional treatments can only expect limited effects, thereby novel therapies are badly in need to promote endometrium regeneration. Here we generated a decellularized extracellular matrix (ECM) from porcine dermis, and composited adipose stem cell derived exosomes (ADSC-exos) on it (ECM@ADSC-exos). In vitro experiments proved that ECM@ADSC-exos exhibited good cytocompatibility and could improve cell proliferation, migration and angiogenesis. We also observed that, when implanted in the uterine cavity of a rat model of endometrium injury, ECM@ADSC-exos improved endometrium regeneration, enhanced local angiogenesis, promoted myometrium repair and finally preserved fertility. Our results proved that ECM@ADSC-exos could be a novel option for endometrium regeneration.