Neutral pH photoenzymatic activity of Au-doped g-C3N4 nanosheet for colorimetric detection of total antioxidant capacity in food samples

Abstract

Total antioxidant capacity (TAC) is vital for food quality evaluation. The emergence of various nanozymes with TMB as substrate offered a new avenue for TAC detection due to simple operation and fast response, but a long-standing challenge is its low activity at physiological pH, which may account for the discrepancy between the measured TAC and the actual antioxidant capacity in vivo. Herein, Au doping was explored to break the pH limitation of g-C₃N₄ nanosheets (CNNS) photozyme. The catalytic activities of Au@CNNS at pH 4.0 and 7.4 were 14.9- and 6.2-fold higher than that of CNNS at pH 4. The neutral pH photozymatic activity (photosensitized oxidation of TMB, oxidase mimic) of Au@CNNS was explored for sensitivity TAC detection (LOD: 1.0 μM TE), which featured more convenient operations and higher sensitivity over the DPPH assay. The proposed Au@CNNS-based photozymatic colorimetric method was explored for accurate detection of TAC in drinks and juices.

Keywords: Au-doped graphitic carbon nitride nanosheets; Colorimetric assay; Neutral pH; Photozymatic activity; Total antioxidant capacity.